miR-30a-5pによって制御される癌促進的分子が膵管腺癌に及ぼす影響

• Material Type: 博士論文
• Title: miR-30a-5pによって制御される癌促進的分子が膵管腺癌に及ぼす影響
• Author/Editor: ネパール, プラモド
• Author Heading: ネパール, プラモド
• Publication, Distribution, etc.: International Institute of Anticancer Research
• Alternative Title: Impact of Oncogenic Targets Controlled by Tumor-Suppressive miR-30a-5p in Pancreatic Ductal Adenocarcinoma
• Degree grantor/type: 鹿児島大学
• Date Granted: 2022-02-17
• Dissertation Number: 甲総研第635号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: microRNA; miR-30a-5p; RRM2; pancreatic ductal adenocarcinoma; tumor-suppressor
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Background/Aim: Our recent miRNA analyses revealed that miR-30a-5p has tumor-suppressive activity in pancreatic ductal adenocarcinoma (PDAC). Herein, we sought to identify tumor-suppressive genes controlled by miR-30a-5p, emphasizing on genes that are closely involved in the molecular pathogenesis of PDAC. We uncovered several novel findings regarding the pathogenesis of this disease. Materials and Methods: In silico analyses were used to identify the putative target genes of miR-30a-5p and assess their expression levels. Direct regulation of RRM2 by miR-30a-5p and its oncogenic functions were evaluated in PDAC cell lines. Overexpression of RRM2 was demonstrated in clinical samples. Results: A total of 24 putative targets were identified by in silico database analysis. High expression of 4 genes (CBFB, RRM2, AHNAK, and DCBLD1) was significantly associated with shorter survival of patients with PDAC. Functional assays demonstrated that knockdown of RRM2 attenuated the malignant phenotype of PDAC cells. Conclusion: The miR-30a-5p/RRM2 axis facilitated the malignant transformation of PDAC cells. PRAMOD NEPAL, YUTO HOZAKA, TAKAKO TANAKA, MASUMI WADA, SHUNICHI ASAI, CHIKASHI MINEMURA, TETSUYA IDICHI, TAKAAKI ARIGAMI, HIROSHI KURAHARA, NAOHIKO SEKI and TAKAO OHTSUKA Impact of Oncogenic Targets Controlled by Tumor-Suppressive miR-30a-5p in Pancreatic Ductal Adenocarcinoma ANTICANCER RESEARCH 41: 4821-4836 (2021) https://doi.org/10.21873/anticanres.15297
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Pramod_Nepal_isk_635 other

Comments_Pramod_Nepal_isk_635 other

Diss_プラモド_ネパール_ISK635_2021 fulltext

Abstract_プラモド_ネパール_4517810437_2021 abstract