エナメル上皮腫細胞と骨芽細胞の細胞間シグナル伝達について

• Material Type: 博士論文
• Title: エナメル上皮腫細胞と骨芽細胞の細胞間シグナル伝達について
• Author/Editor: ハイラニ, エリサ
• Author Heading: ハイラニ, エリサ
• Publication, Distribution, etc.: Elsevier
• Alternative Title: Intercellular Siganling between Ameloblastoma and Osteoblasts
• Degree grantor/type: 鹿児島大学
• Date Granted: 2022-04-20
• Dissertation Number: 甲総研第657号
• Degree Type: 博士(歯学); Doctor of Philosophy in Dental Science
• Text Language Code: eng
• Subject Heading: Ameloblastoma; AM-3; Osteoblast; Tumor-bone microenvironment; Cytokine; Intercellular communication
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Ameloblastoma is an odontogenic tumor located in the bone jaw with clinical characteristics of extensive bone resorption. It is a locally invasive tumor with a high recurrence rate despite adequate surgical removal. In bone disease, tumors and other cells including osteoblasts, osteoclasts, and osteocytes in the bone microenvironment contribute to the pathogenesis of tumor growth. However, the effect of osteoblasts on ameloblastoma cells is not well-understood, and there has been limited research on interactions between them. This study investigated interactions between ameloblastoma cells and osteoblasts using a human ameloblastoma cell line (AM-3 ameloblastoma cells) and a murine pre-osteoblast cell line (MC3T3-E1 cells). We treated each cell type with the conditioned medium by the other cell type. We analyzed the effect on cytokine production by MC3T3-E1 cells and the production of MMPs by AM-3 cells. Treatment with AM-3-conditioned medium induced inflammatory cytokine production of IL-6, MCP-1, and RANTES from MC3T3-E1 cells. The use of an IL-1 receptor antagonist suppressed the production of these inflammatory cytokines by MC3T3-E1 cells stimulated with AM-3-conditioned medium. The MC3T3-E1-conditioned medium triggered the expression of MMP-2 from AM-3 cells. Furthermore, we have shown that the proliferation and migration activity of AM-3 cells were accelerated by MC3T3-E1 conditioned media. In conclusion, these intercellular signalings between ameloblastoma cells and osteoblasts may play multiple roles in the pathogenesis of ameloblastoma. Elissa Chairani, Takao Fuchigami, Hirofumi Koyama, Yusuke Ono, Mikio Iijima, Michiko Kishida, Toshiro Kibe, Norifumi Nakamura, Shosei Kishida Intercellular signaling between ameloblastoma and osteoblasts Biochemistry and Biophysics Reports 30 (2022) 101233 https://doi.org/10.1016/j.bbrep.2022.101233
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_Elissa_Chairani_isk_657 other

Comments_Elissa_Chairani_isk_657 other

Diss_エリサ_ハイラニ_ISK657_2022 fulltext

Abstract_エリサ_ハイラニ_4518810404_2022 abstract