小細胞肺癌および非小細胞肺癌細胞由来の細胞外小胞におけるN結合型糖鎖構造の違いの同定

• Material Type: 博士論文
• Title: 小細胞肺癌および非小細胞肺癌細胞由来の細胞外小胞におけるN結合型糖鎖構造の違いの同定
• Author/Editor: 近藤, 清貴
• Author Heading: 近藤, 清貴 コンドウ, キヨタカ
• Publication, Distribution, etc.: American Society for Biochemistry and Molecular Biology
• Alternative Title: Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells
• Degree grantor/type: 鹿児島大学
• Date Granted: 2022-11-17
• Dissertation Number: 甲総研第676号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Text Language Code: eng
• Subject Heading: exosome (vesicle); extracellular vesicles; glycomics; glycosylation; integrins; lung cancers
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Asparagine-linked glycosylation (N-glycosylation) of proteins in the cancer secretome has been gaining increasing attention as a potential biomarker for cancer detection and diagnosis. Small extracellular vesicles (sEVs) constitute a large part of the cancer secretome, yet little is known about whether their N-glycosylation status reflects known cancer characteristics. Here, we investigated the N-glycosylation of sEVs released from small-cell lung carcinoma (SCLC) and non–small-cell lung carcinoma (NSCLC) cells. We found that the N-glycans of SCLC-sEVs were characterized by the presence of structural units also found in the brain N-glycome, while NSCLC-sEVs were dominated by typical lung-type N-glycans with NSCLC-associated core fucosylation. In addition, lectin-assisted N-glycoproteomics of SCLC-sEVs and NSCLC-sEVs revealed that integrin αV was commonly expressed in sEVs of both cancer cell types, while the epithelium-specific integrin α6β4 heterodimer was selectively expressed in NSCLC-sEVs. Importantly, N-glycomics of the immunopurified integrin α6 from NSCLC-sEVs identified NSCLC-type N-glycans on this integrin subunit. Thus, we conclude that protein N-glycosylation in lung cancer sEVs may potentially reflect the histology of lung cancers. Kiyotaka Kondo, Yoichiro Harada, Miyako Nakano, Takehiro Suzuki, Tomoko Fukushige, Ken Hanzawa, Hirokazu Yagi, Koichi Takagi, Keiko Mizuno, Yasuhide Miyamoto, Naoyuki Taniguchi, Koichi Kato, Takuro Kanekura, Naoshi Dohmae, Kentaro Machida, Ikuro Maruyama, Hiromasa Inoue Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non–small-cell lung cancer cells Journal of Biological Chemistry (2022) 298(6) 101950 https://doi.org/10.1016/j.jbc.2022.101950
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ

Table of Contents

Result_KONDO_ Kiyotaka_isk_676 other

Comments_KONDO_kiyotaka_isk_676 other

Diss_近藤_清貴_ISK676_2022 fulltext

Abstract_近藤_清貴_4516810129_2022 abstract