うがい液中のエクソソーム由来マイクロRNAは、シェーグレン症候群の新規バイオマーカーとしての可能性を持つ

Material type: 博士論文

Author: 山城, 康太

Publisher: MDPI

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Material Format: Digital

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Name of awarding university/degree: 鹿児島大学,博士(歯学),Doctor of Philosophy in Dental Science

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Note (General)：: 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨Sjögren syndrome (SS) is diagnosed based on invasive tissue biopsies and blood sampling. Therefore, a novel non-inva...

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Material Type: 博士論文
Title: うがい液中のエクソソーム由来マイクロRNAは、シェーグレン症候群の新規バイオマーカーとしての可能性を持つ
Author/Editor: 山城, 康太
Author Heading: 山城, 康太ヤマシロ, コウタ
Publication, Distribution, etc.: MDPI
Alternative Title: Exosome-Derived microRNAs from Mouthrinse Have the Potential to Be Novel Biomarkers for Sjögren Syndrome
Degree grantor/type: 鹿児島大学
Date Granted: 2023-02-15
Dissertation Number: 甲総研第681号
Degree Type: 博士(歯学)
Doctor of Philosophy in Dental Science
Text Language Code: eng
Subject Heading: Sjögren syndrome
mouthrinse
microRNA
exosome
liquid biopsy
diagnosis
screening test
oral science
Target Audience: 一般
Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨
Sjögren syndrome (SS) is diagnosed based on invasive tissue biopsies and blood sampling. Therefore, a novel non-invasive and simple inspection diagnostic marker of SS is required. Here, we identified exosome-derived microRNAs (miRNAs) as biomarkers for SS using non-invasive mouthrinse samples collected from patients with SS and healthy volunteers. We compared miRNAs derived from exosomes in mouthrinse samples from the two groups using microarrays and real-time polymerase chain reaction (PCR) and identified 12 miRNAs as biomarker candidates. The expression ratios of four miRNAs were significantly increased in the SS group compared to the control group. Logistic regression analysis revealed a more significant influence of miR-1290 and let-7b-5p in the SS group than that in the control group. We combined these miRNAs to create a diagnostic prediction formula using logistic regression analysis. The combination of miR-1290 and let-7b-5p distinguished SS from the control samples with an AUC, sensitivity, specificity, positive predictive value, and negative predictive value of 0.856, 91.7%, 83.3%, 84.6%, and 90.9%, respectively. These results indicated that an increased ratio of these miRNAs could serve as a novel and non-invasive diagnostic marker for SS. This is the first report of diagnosis and screening of SS by adopting a non-invasive method using mouthrinse. Kouta Yamashiro, Tomofumi Hamada, Kazuki Mori, Keitaro Nishi, Maya Nakamura, Mahiro Beppu, Akihiko Tanaka, Hiroshi Hijioka, Yoshiaki Kamikawa and Tsuyoshi Sugiura Exosome-Derived microRNAs from Mouthrinse Have the Potential to Be Novel Biomarkers for Sjögren Syndrome Journal of Personalized Medicine 2022, 12, 1483 https://doi.org/10.3390/jpm12091483
Format (IMT): application/pdf
Source: Diss_山城_康太_ISK681_2022 （fulltext）
https://ir.kagoshima-u.ac.jp/record/16477/files/Diss_%E5%B1%B1%E5%9F%8E_%E5%BA%B7%E5%A4%AA_ISK681_2022.pdf
Access Restrictions: インターネット公開
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ