新興下痢症病原体Escherichia albertiiにおける鞭毛H抗原の多様性と遺伝子型別法の開発

Material type: 博士論文

Author: 中江, 広治

Publisher: Frontiers Media SA

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Material Format: Digital

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Name of awarding university/degree: 鹿児島大学,博士(医学),Doctor of Philosophy in Medical Science

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Note (General)：: 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli. As E...

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Material Type: 博士論文
Title: 新興下痢症病原体Escherichia albertiiにおける鞭毛H抗原の多様性と遺伝子型別法の開発
Author/Editor: 中江, 広治
Author Heading: 中江, 広治ナカエ, コウジ
Publication, Distribution, etc.: Frontiers Media SA
Alternative Title: Diversification of Escherichia albertii H-antigens and Development of H-Genotyping PCR
Degree grantor/type: 鹿児島大学
Date Granted: 2023-03-10
Dissertation Number: 甲総研第696号
Degree Type: 博士(医学)
Doctor of Philosophy in Medical Science
Text Language Code: eng
Subject Heading: Escherichia albertii
flagellin
fliC
H-antigen
genotyping
Target Audience: 一般
Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨
Escherichia albertii is a recently recognized human enteropathogen that is closely related to Escherichia coli. As E. albertii sometimes causes outbreaks of gastroenteritis, rapid strain typing systems, such as the O- and H-serotyping systems widely used for E. coli, will be useful for outbreak investigation and surveillance. Although an O-genotyping system has recently been developed, the diversity of E. albertii H-antigens (flagellins) encoded by fliC genes remains to be systematically investigated, and no H-serotyping or genotyping system is currently available. Here, we analyzed the fliC genes of 243 genome-sequenced E. albertii strains and identified 73 sequence types, which were grouped into four clearly distinguishable types designated E. albertii H-genotypes 1–4 (EAHg1–EAHg4). Although there was a clear sign of intraspecies transfer of fliC genes in E. albertii, none of the four E. albertii H-genotypes (EAHgs) were closely related to any of the 53 known E. coli H-antigens, indicating the absence or rare occurrence of interspecies transfer of fliC genes between the two species. Although the analysis of more E. albertii strains will be required to confirm the low level of variation in their fliC genes, this finding suggests that E. albertii may exist in limited natural hosts or environments and/or that the flagella of E. albertii may function in a limited stage(s) in their life cycle. Based on the fliC sequences of the four EAHgs, we developed a multiplex PCR-based H-genotyping system for E. albertii (EAH-genotyping PCR), which will be useful for epidemiological studies of E. albertii infections. Koji Nakae, Tadasuke Ooka, Koichi Murakami, Yukiko Hara-Kudo, Naoko Imuta, Yasuhiro Gotoh, Yoshitoshi Ogura, Tetsuya Hayashi, Yasuhiro Okamoto and Junichiro Nishi Diversification of Escherichia albertii H-Antigens and Development of H-Genotyping PCR Frontiers in Microbiology. 12:737979 (2021) https://doi.org/10.3389/fmicb.2021.737979
Format (IMT): application/pdf
Source: Diss_中江_広治_ISK696_2022 （fulltext）
https://ir.kagoshima-u.ac.jp/record/16540/files/Diss_%E4%B8%AD%E6%B1%9F_%E5%BA%83%E6%B2%BB_ISK696_2022.pdf
Access Restrictions: インターネット公開
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 鹿児島大学 : 鹿児島大学リポジトリ