Electrophoretic separation of cells and particles from rat pituitary (Pituitary)

• Material Type: 規格・テクニカルリポート類
• Title: Electrophoretic separation of cells and particles from rat pituitary (Pituitary)
• Author/Editor: Hymer, W.C.; Salada, T.; Cenci, R.; Krishnan, K.; Seaman, G.; 長岡, 俊治; Hymer, W. C.; Nagaoka, Shunji
• Author Heading: Hymer, W.C.; Salada, T.; Cenci, R.; Krishnan, K.; Seaman, G.; 長岡, 俊治; Hymer, W. C.; Nagaoka, Shunji
• Publication, Distribution, etc.: 宇宙開発事業団
• Publication Date: 1996-03-15
• Publication Date (W3CDTF): 1996-03-15
• Alternative Title: 電気泳動によるラット脳下垂体からの細胞と粒子の分離(PITUITARY)
• Periodical title: 宇宙開発事業団技術報告
• Pages: 205-220
• ISSN (Periodical Title): ISSN : 1345-7888
• Text Language Code: eng
• Subject Heading: ラット; 脳下垂体; GH; 成長ホルモン; CFE; 連続流電気泳動; 微小重力; 培養細胞; 宇宙実験; 粒子; rat; pituitary; growth hormone; continuous flow electrophoresis; microgravity; cultured cell; space experiment; particle
• Target Audience: 一般
• Note (General): This report describes the results of a Continuous Flow Electrophoresis (CFE) experiment done on STS-65 in which the idea that intracellular Growth Hormone (GH) particles contained in a cell lysate prepared from cultured rat anterior pituitary cells in microgravity might have different electrophoretic mobilities from those in a synchronous ground control cell lysate was tested. Collectively, the results suggested that CFE processing in microgravity was better than on earth; more sample could be processed/time (6x) and more variant forms of GH molecules could be resolved as well. The doing of a pituitary cell CFE experiment had been also hoped, but failure of the hardware required that the actual cell electrophoresis trials be done on earth shortly after Shuttle landing. Data from these experiments showed that spaceflown cells were more electrophoretically mobile than ground control cells, thereby offering evidence for the idea that exposure of cultured cells to microgravity can change their net surface charge, especially when the cells are fed. Collectively, the results from this pituitary cell experiment document the advantage of using coupled cell culture and CFE techniques in the microgravity environment.; 資料番号: AA0000271010; レポート番号: NASDA-TMR-960004
• Access Restrictions: インターネット公開
• Periodical Title (NCID): AN00364784; https://ci.nii.ac.jp/ncid/AN00364784
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 宇宙航空研究開発機構 : 宇宙航空研究開発機構リポジトリ

• Summary, etc.: 本稿ではSTS-65でおこなわれた連続流電気泳動(CFE)実験の結果を述べる。微小重力下で、ラット培養前脳下垂体細胞から調製された細胞溶解産物に含まれる成長ホルモン(GH)粒子は、同一速度で移動する地上対照細胞溶解産物とは違った電気泳動移動度を示す可能性があると考え、実験をおこなった。微小重力下では...