Mesenchymal stem cells ameliorate experimental peritoneal fibrosis by suppressing inflammation and inhibiting TGF-β1 signaling

• Material Type: 博士論文
• Title: Mesenchymal stem cells ameliorate experimental peritoneal fibrosis by suppressing inflammation and inhibiting TGF-β1 signaling
• Author/Editor: Ueno, Toshinori
• Author Heading: Ueno, Toshinori
• Alternative Title: 間葉系幹細胞は炎症抑制およびTGF-β1シグナルの抑制を介し腹膜線維化を改善させる
• Degree grantor/type: 広島大学(Hiroshima University)
• Date Granted: 2013-05-23
• Dissertation Number: 甲第6191号
• Degree Type: 博士(医学)
• Text Language Code: eng
• Subject Heading: epithelial-to-mesenchymal transition; hepatocyte growth factor; mesenchymal stem cells; peritoneal fibrosis; transforming growth factor-β1
• NDC: 490
• Target Audience: 一般
• Note (General): type:text; Mesenchymal stem cells (MSCs) are multipotent adult stem cells that have regenerative capability and exert paracrine actions on damaged tissues. Since peritoneal fibrosis is a serious complication of peritoneal dialysis, we tested whether MSCs suppress this using a chlorhexidine gluconate model in rats. Although MSCs isolated from green fluorescent protein? positive rats were detected for only 3 days following their injection, immunohistochemical staining showed that MSCs suppressed the expression of mesenchymal cells, their effects on the deposition of extracellular matrix proteins, and the infiltration of macrophages for 14 days. Moreover, MSCs reduced the functional impairment of the peritoneal membrane. Cocultures of MSCs and human peritoneal mesothelial cells using a Transwell system indicated that the beneficial effects of MSCs on the glucose-induced upregulation of transforming growth factor-β1(TGF-β1) and fibronectin mRNA expression in the human cells were likely due to paracrine actions. Preincubation in MSC-conditioned medium suppressed TGFβ1- induced epithelial-to-mesenchymal transition, a-smooth muscle actin, and the decrease in zonula occludens-1 in cultured human peritoneal mesothelial cells. Although bone morphogenic protein 7 was not detected, MSCs secreted hepatocyte growth factor and a neutralizing antibody to this inhibited TGF-β1 signaling. Thus, our findings imply that MSCs ameliorate experimental peritoneal fibrosis by suppressing inflammation and TGF-β1 signaling in a paracrine manner.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): Copyright(c) by Author
• References (URI): http://dx.doi.org/10.1038/ki.2013.81; URI
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 広島大学 : 広島大学学術情報リポジトリ

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http://ir.lib.hiroshima-u.ac.jp/files/public/3/36259/20141121004829710370/k6191_3.pdf fulltext

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