Oxidative stress regulates expression of claudin-1 in human RPE cells

Material type: 博士論文

Author: Hirata, Junko

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Name of awarding university/degree: 広島大学(Hiroshima University),博士(医学)

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Note (General)：: type:textAge-related macular degeneration (AMD) is a neurodegenerative disease associated with irreversible loss of central vision in the elderly. Dis...

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Material Type: 博士論文
Title: Oxidative stress regulates expression of claudin-1 in human RPE cells
Author/Editor: Hirata, Junko
Author Heading: Hirata, Junko
Alternative Title: 酸化ストレスによる網膜色素上皮細胞内の接着蛋白質claudin-1の発現変化
Degree grantor/type: 広島大学(Hiroshima University)
Date Granted: 2013-11-28
Dissertation Number: 甲第6272号
Degree Type: 博士(医学)
Text Language Code: eng
Subject Heading: Age-related macular degeneration
Retinal pigment epithelium
Oxidative stress
Claudin-1
Tight junction
p38
Mitogen-activated protein kinase
NDC: 490
Target Audience: 一般
Note (General): type:text
Age-related macular degeneration (AMD) is a neurodegenerative disease associated with irreversible loss of central vision in the elderly. Disruption of the homeostatic function of the retinal pigment epithelium (RPE) is thought to be fundamental to AMD pathogenesis, and oxidative stress is implicated in the associated RPE damage. We examined the effects of oxidative stress on the expression of junctional proteins in cultured human retinal pigment epithelial (ARPE-19) cells. Reverse transcription-PCR and immunoblot analyses revealed that expression of the tight-junction protein claudin-1 was increased at both the mRNA and protein levels 8 to 12 h after exposure of ARPE-19 cells to H2O2, whereas that of the tight-junction protein ZO-1 or the adherens-junction protein N-cadherin was unaffected. Expression of both claudin-1 and N-cadherin was down-regulated by exposure of the cells to H2O2 for longer periods (24 to 48 h). Oxidative stress also induced the phosphorylation of p38 mitogen-activated protein kinase (MAPK) with a time course similar to that apparent for the up-regulation of claudin-1 expression. Furthermore, the increase in the abundance of claudin-1 induced by H2O2 was blocked by the p38 inhibitor SB203580. Phosphorylation of the MAPKs ERK and JNK was not affected by H2O2. Our results suggest that modulation of claudin-1 expression in the RPE by oxidative stress may contribute to the pathogenesis of AMD.
Format (IMT): application/pdf
Source: fulltext
https://ir.lib.hiroshima-u.ac.jp/files/public/4/40614/20160726095643567848/k6272_3.pdf
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https://ir.lib.hiroshima-u.ac.jp/files/public/4/40614/20181121143903343104/k6272_1.pdf
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https://ir.lib.hiroshima-u.ac.jp/files/public/4/40614/20181121143903838760/k6272_2.pdf
Access Restrictions: インターネット公開
References (URI): URI
http://doi.org/10.2478/s11535-014-0287-0
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 広島大学 : 広島大学学術情報リポジトリ