Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes

Digital data available（秋田大学学術情報リポジトリ）

学術機関リポジトリデータベース（IRDB）（機関リポジトリ）

Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes

Material type: 博士論文

Author: フローレス, マリアジョリナルーほか

Publisher: -

Publication date: 2014-06-01

Material Format: Digital

Capacity, size, etc.: -

Name of awarding university/degree: 秋田大学,博士（医学）

View All

Notes on use

Note (General)：: Aim: Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism...

Related materials as well as pre- and post-revision versions

http://hdl.handle.net/10295/2507

Search by Bookstore

Holdings of Libraries in Japan

This page shows libraries in Japan other than the National Diet Library that hold the material.

List of Cooperating Institutions and Databases

Please contact your local library for information on how to use materials or whether it is possible to request materials from the holding libraries.

other

Akita University Institutional Repository
Digital
You can check the holdings of institutions and databases with which Institutional Repositories DataBase(IRDB)(Institutional Repository) is linked at the site of Institutional Repositories DataBase(IRDB)(Institutional Repository).
Akita University Institutional Repository

Search by Bookstore

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 博士論文
Title: Characterization of glycosylphosphatidylinositol-anchored ceruloplasmin enriched in the apical plasma membrane of rat hepatocytes
Author/Editor: フローレス, マリアジョリナルー
Flores, Maria Jolina Lou
Author Heading: フローレス, マリアジョリナルー
Flores, Maria Jolina Lou
Publication Date: 2014-06-01
Publication Date (W3CDTF): 2014-06-01
Alternative Title: ラット肝細胞の頂端面側細胞膜に局在するGPI-セルロプラスミンの特性に関する研究
Degree grantor/type: 秋田大学
Date Granted: 2014-03-22
Dissertation Number: 甲第1088号
Degree Type: 博士（医学）
Text Language Code: eng
Subject Heading: apical membrane
ceruloplasmin
GPI-Cp
hepatocyte
Target Audience: 一般
Note (General): Aim: Ceruloplasmin (Cp) is an acute-phase protein and a member of the multicopper oxidase family of enzymes. It has been implicated in iron metabolism because of its ferroxidase activity. It is expressed as soluble (sCp) or glycosylphosphatidylinositolanchored ceruloplasmin (GPI-Cp) form; the former is primarily synthesized in the liver, and the latter is primarily found in the brain. Although recent studies reported GPI-Cp expression on hepatocytes, little is known regarding its presence in specific liver cell compartments and its possible involvement in liver pathophysiology. This study aimed to characterize the distribution of GPI-Cp in liver cells and specifically in the apical part of the plasma membrane. Methods: We assessed GPI-Cp expression in the liver using immunohistochemistry and immunoblotting techniques. Furthermore, we isolated apical and basolateral membrane fraction from the total liver membrane using sucrose discontinuous gradient centrifugation, and GPI-Cp were detected using immunoblotting. Results: GPI-Cp was detected in purified apical membranes of rat liver cells. Immunoreactive Cp protein was released after incubation with phosphatidylinositolspecific phospholipase C, and the free protein demonstrated ferroxidase activity. Conclusion: These findings suggest that majority of GPI-Cp present in the liver is primarily located on the apical surface of cells because of transcytosis.
Access Restrictions: インターネット公開
Related Material: http://hdl.handle.net/10295/2507
http://hdl.handle.net/10295/2507
Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
https://irdb.nii.ac.jp
Original Data Provider (Database): 秋田大学 : 秋田大学学術情報リポジトリ