C-Reactive Protein Reduces the Relative Number of Tumor -Associated M2 Macrophages and ntratumoral Angiogenesis in Mice

• Material Type: 博士論文
• Title: C-Reactive Protein Reduces the Relative Number of Tumor -Associated M2 Macrophages and ntratumoral Angiogenesis in Mice
• Author/Editor: 栗林, 邦明; KURIBAYASHI, Kuniaki
• Author Heading: 栗林, 邦明; KURIBAYASHI, Kuniaki
• Publication Date: 2015-06-01
• Publication Date (W3CDTF): 2015-06-01
• Alternative Title: CRP の抗腫瘍効果 -腫瘍関連マクロファージと腫瘍内血管新生に関して-
• Degree grantor/type: 秋田大学
• Date Granted: 2015-03-22
• Dissertation Number: 甲第1116号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Angiogenesis; Colony stimulating factor 1; C-reactive protein; M2 phenotype; Tumor-associated; macrophages
• Target Audience: 一般
• Note (General): Tumor-associated macrophages play a key role in cancer metastasis. On the other hand, Creactive protein (CRP), a widely used biomarker of inflammation, has been shown to have inhibitory effects on tumor proliferation and metastasis. Here we used an implanted tumor mouse model to assess the effect of CRP on tumor-associated macrophage numbers and on their phenotype, as well as on intratumoral angiogenesis. NR-S1M murine oral squamous cell carcinoma cells were implanted subcutaneously in the backs of anesthetized C3H/HeN mice. Some of the mice were also subcutaneously administered 1 μg of recombinant mouse CRP in 100 μL of phosphate-buffered saline (PBS) (CRP group, n = 10) near the neck every 2 days for 30 days (15 injections in all). Control mice received PBS without CRP. The mice were then sacrificed and the excised tumors were analyzed. Tumor weight and size did not differ between the two groups, but immunohistochemical analysis showed the F4/80+ macrophage (total macrophages) count to be significantly larger in the CRP group (P = 0.0028), while the relative number of CD206+ anti-inflammatory M2 macrophages was significantly reduced (P = 0.0091). In addition, expression of colony stimulating factor 1 mRNA, which is associated with the M2 macrophage phenotype, was significantly lower in the CRP group. Intratumoral angiogenesis, indicated by the presence of CD31+ vessels within the tumor, was reduced in the CRP group (P = 0.0028). These findings suggest that CRP has therapeutic potential against cancer through decreasing the accumulation of M2 macrophages and angiogenesis within tumors.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): 東北ジャーナル刊行会
• Related Material (DOI): 10.1620/tjem.233.249
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 秋田大学 : 秋田大学学術情報リポジトリ

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