マカクの付着系細胞におけるin vitro加齢変化

• 資料種別: 記事
• タイトル: マカクの付着系細胞におけるin vitro加齢変化
• 著者・編者: 清水 裕子
• 著者標目: 清水 裕子
• タイトル（掲載誌）: 霊長類研究 = Primate research
• 巻号年月日等（掲載誌）: 16(2) 2000.10
• 掲載巻: 16
• 掲載号: 2
• 掲載ページ: 77～85
• 掲載年月日（W3CDTF）: 2000-10
• ISSN（掲載誌）: 0912-4047
• ISSN-L（掲載誌）: 0912-4047
• 出版事項（掲載誌）: 東京 : 日本霊長類学会
• 出版地（国名コード）: JP
• 本文の言語コード: jpn
• NDLC: ZR4
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z18-1898
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 5537220; http://id.ndl.go.jp/bib/5537220
• 整理区分コード: 632

• 要約等: Human fibroblasts have a limited replicative lifespan and never undergo infinite cell division <i>in vitro</i>. In contrast, rodent fibroblasts spontaneously and highly frequently immortalize <i>in vitro</i>. Therefore, rodent is inappropriate as a model animal to study human aging <i>in vitro</i>. To test effects of macaque monkey as the model system, macaque adherent cells were cultured and passaged <i>in vitro</i> and analyzed cytologically.<br>Long-tailed macaque (<i>Macaca fascicularis</i>), Japanese macaque (<i>Macaca fuscata</i>), and bonnet monkey (<i>Macaca radiata</i>) were subjected to the study. Adherent cells were isolated from their skin, kidney, and lung. A total of 19 cell cultures were examined until terminating cell division.<br>Most of the cultures (17/19) exhibited senescence by 7-25 Population Doubling Levels (PDLs), showing enlargement of cell size, decrease of saturation density and extension of doubling interval, and then terminated cell division [Mortality stage 1 (M1)]. The remaining two cell cultures showed distinct pattern. They first exhibited senescent morphology at around 20PDLs, but continued cell division through M1 up to 106 PDLs and then went into crisis stage [Mortality stage 2 (M2)]. In all cell cultures tested, telomerase activity was not detected. Consistently, telomeres appeared to be shortened by every PDLs.<br>Macaque cells showed an intermediate pattern of <i>in vitro</i> aging between human and rodent cells, whereas, they showed no telomerase activity similarly to human cells. Therefore, the macaques must serve as an excellent animal model to study human cellular aging and provide us with a key to study the mechanism of the transition to critical stages in cellular aging.
• DOI: 10.2354/psj.16.77
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: Human fibroblasts have a limited replicative lifespan and never undergo infinite cell division <i>in vitro</i>. In contrast, rodent fibroblasts spontaneously and highly frequently immortalize <i>in vitro</i>. Therefore, rodent is inappropriate as a model animal to study human aging <i>in vitro</i>. To test effects of macaque monkey as the model system, macaque adherent cells were cultured and passaged <i>in vitro</i> and analyzed cytologically.<br>Long-tailed macaque (<i>Macaca fascicularis</i>), Japanese macaque (<i>Macaca fuscata</i>), and bonnet monkey (<i>Macaca radiata</i>) were subjected to the study. Adherent cells were isolated from their skin, kidney, and lung. A total of 19 cell cultures were examined until terminating cell division.<br>Most of the cultures (17/19) exhibited senescence by 7-25 Population Doubling Levels (PDLs), showing enlargement of cell size, decrease of saturation density and extension of doubling interval, and then terminated cell division [Mortality stage 1 (M1)]. The remaining two cell cultures showed distinct pattern. They first exhibited senescent morphology at around 20PDLs, but continued cell division through M1 up to 106 PDLs and then went into crisis stage [Mortality stage 2 (M2)]. In all cell cultures tested, telomerase activity was not detected. Consistently, telomeres appeared to be shortened by every PDLs.<br>Macaque cells showed an intermediate pattern of <i>in vitro</i> aging between human and rodent cells, whereas, they showed no telomerase activity similarly to human cells. Therefore, the macaques must serve as an excellent animal model to study human cellular aging and provide us with a key to study the mechanism of the transition to critical stages in cellular aging.
• DOI: 10.2354/psj.16.77; https://doi.org/10.2354/psj.16.77
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（URI）: http://id.ndl.go.jp/bib/5537220; https://ndlsearch.ndl.go.jp/books/R000000004-I5537220
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• 書誌ID（NDLBibID）: 5537220; http://id.ndl.go.jp/bib/5537220
• NII論文ID: 10010164979