PLIN5 Suppresses Lipotoxicity and Ferroptosis in Cardiomyocyte via Modulating PIR/NF-κB Axis

• 資料種別: 記事
• タイトル: PLIN5 Suppresses Lipotoxicity and Ferroptosis in Cardiomyocyte via Modulating PIR/NF-κB Axis
• 著者標目: Shen Xiaoyu; Zhou Zhou; Zhang Jiamei; Yu Ruiqun
• 出版年月日等: 2024-05-31
• 出版年（W3CDTF）: 2024-05-31
• タイトル（掲載誌）: International Heart Journal
• 巻号年月日等（掲載誌）: 65 3
• 掲載巻: 65
• 掲載号: 3
• 掲載ページ: 537-547
• 掲載年月日（W3CDTF）: 2024-05-31
• ISSN（掲載誌）: 13492365
• ISSN-L（掲載誌）: 13492365
• 出版事項（掲載誌）: International Heart Journal Association
• 本文の言語コード: en
• 件名標目: Diabetic cardiomyopathy; Inflammation; NF-κB pathway; Cardiomyocyte lipotoxicity; Iron metabolism; Cell death
• 対象利用者: 一般
• 標準番号（その他）: PMID : 38749744
• DOI: 10.1536/ihj.24-002; https://doi.org/10.1536/ihj.24-002
• 関連情報（URI）: https://www.jstage.jst.go.jp/article/ihj/65/3/65_24-002/_pdf; https://search.jamas.or.jp/link/ui/2025191061
• 参照: Pyrin Inflammasome Regulates Tight Junction Integrity to Restrict Colitis and Tumorigenesis; https://cir.nii.ac.jp/crid/1360011145017699968; Inflammasomes in neuroinflammatory and neurodegenerative diseases; https://cir.nii.ac.jp/crid/1360011145111184256; Plin5, a New Target in Diabetic Cardiomyopathy; https://cir.nii.ac.jp/crid/1360018990388420352; Copper-dependent autophagic degradation of GPX4 drives ferroptosis; https://cir.nii.ac.jp/crid/1360018990785566208; Effects of Sodium/Glucose Cotransporter 2 (SGLT2) Inhibitors on Cardiovascular and Metabolic Outcomes in Patients Without Diabetes Mellitus: A Systematic Review and Meta‐Analysis of Randomized‐Controlled Trials; https://cir.nii.ac.jp/crid/1360018992597847168; Role of Dapagliflozin and Liraglutide on Diabetes-Induced Cardiomyopathy in Rats: Implication of Oxidative Stress, Inflammation, and Apoptosis; https://cir.nii.ac.jp/crid/1360018992713480192; Electroacupuncture inhibits the interaction between peripheral TRPV1 and P2X3 in rats with different pathological pain; https://cir.nii.ac.jp/crid/1360018992719680512; Ferroptosis as a novel therapeutic target for cardiovascular disease; https://cir.nii.ac.jp/crid/1360018993020253312; Role of Oxidative Stress in Metabolic and Subcellular Abnormalities in Diabetic Cardiomyopathy; https://cir.nii.ac.jp/crid/1360018993045866368; Perilipin5 protects against lipotoxicity and alleviates endoplasmic reticulum stress in pancreatic β-cells; https://cir.nii.ac.jp/crid/1360018993069702656; The role of cardiac lipotoxicity in the pathogenesis of diabetic cardiomyopathy; https://cir.nii.ac.jp/crid/1360018994447226368; Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element; https://cir.nii.ac.jp/crid/1360018994984438272; Ferroptosis and its potential role in the physiopathology of Parkinson’s Disease; https://cir.nii.ac.jp/crid/1360294811398381440; Cardiac Energy Metabolism in Heart Failure; https://cir.nii.ac.jp/crid/1360298344752162432; Hepatic PLIN5 signals via SIRT1 to promote autophagy and prevent inflammation during fasting; https://cir.nii.ac.jp/crid/1360298344799889280; Mir-675-5p supports hypoxia-induced drug resistance in colorectal cancer cells; https://cir.nii.ac.jp/crid/1360300465769560576; TRIM52 plays an oncogenic role in ovarian cancer associated with NF-kB pathway; https://cir.nii.ac.jp/crid/1360300465972266496; Diabetic cardiomyopathy - A comprehensive updated review; https://cir.nii.ac.jp/crid/1360300466729801088; Inhibition of ferroptosis by up-regulating Nrf2 delayed the progression of diabetic nephropathy; https://cir.nii.ac.jp/crid/1360300467583114240; Perilipin5 protects against non-alcoholic steatohepatitis by increasing 11-Dodecenoic acid and inhibiting the occurrence of ferroptosis; https://cir.nii.ac.jp/crid/1360300467682485120; What is the impact of ferroptosis on diabetic cardiomyopathy: a systematic review; https://cir.nii.ac.jp/crid/1360300468730331520; Iron accumulation and lipid peroxidation: implication of ferroptosis in diabetic cardiomyopathy; https://cir.nii.ac.jp/crid/1360300468731584512; 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• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; Crossref; https://www.crossref.org; PubMed; https://pubmed.ncbi.nlm.nih.gov

• 要約等: <p>Cardiomyocyte lipotoxicity and ferroptosis are the key to the development of diabetic cardiomyopathy (DCM). Perilipin 5 (PLIN5) is perceived as a significant target of DCM. This study aimed to focus on the role and mechanism of PLIN5 on lipotoxicity and ferroptosis in DCM.</p><p>Following transfection, mouse cardiomyocytes HL-1 were induced by 0.1 mM palmitic acid (PA) to set up lipotoxic cardiomyocyte models. The cell viability and lipid accumulation were evaluated by cell counting kit-8 assay and Oil red O staining, respectively. Ferrous ion (Fe²⁺), glutathione (GSH), malondialdehyde (MDA), and reactive oxygen species (ROS) levels were determined to verify the effects of PLIN5 or Pirin (PIR) on ferroptosis. Quantitative real-time reverse transcription polymerase chain reaction or Western blot was performed for quantitative analysis.</p><p>PLIN5 overexpression promoted the viability, GSH level, and expression of GPX4/PIR/intracellular P65, yet suppressed lipid accumulation, level of Fe²⁺/MDA/ROS, and expression of interleukin (IL)-1β/IL-18/intranuclear P65 in PA-stimulated HL-1 cells. PIR silencing counteracted the roles of PLIN5 overexpression in PA-stimulated HL-1 cells.</p><p>PLIN5 suppresses lipotoxicity and ferroptosis in cardiomyocyte via modulating PIR/NF-κB axis, hinting its potential as a therapeutic target in DCM.</p>
• DOI: 10.1536/ihj.24-002
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp