PLIN5 Suppresses Lipotoxicity and Ferroptosis in Cardiomyocyte via Modulating PIR/NF-κB Axis

• Material Type: 記事
• Title: PLIN5 Suppresses Lipotoxicity and Ferroptosis in Cardiomyocyte via Modulating PIR/NF-κB Axis
• Author Heading: Shen Xiaoyu; Zhou Zhou; Zhang Jiamei; Yu Ruiqun
• Publication Date: 2024-05-31
• Publication Date (W3CDTF): 2024-05-31
• Periodical title: International Heart Journal
• No. or year of volume/issue: 65 3
• Volume: 65
• Issue: 3
• Pages: 537-547
• Publication date of volume/issue (W3CDTF): 2024-05-31
• ISSN (Periodical Title): 13492365
• ISSN-L (Periodical Title): 13492365
• Publication (Periodical Title): International Heart Journal Association
• Text Language Code: en
• Subject Heading: Diabetic cardiomyopathy; Inflammation; NF-κB pathway; Cardiomyocyte lipotoxicity; Iron metabolism; Cell death
• Target Audience: 一般
• Standard No. (Other): PMID : 38749744
• DOI: 10.1536/ihj.24-002; https://doi.org/10.1536/ihj.24-002
• Related Material (URI): https://www.jstage.jst.go.jp/article/ihj/65/3/65_24-002/_pdf; https://search.jamas.or.jp/link/ui/2025191061
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• Data Provider (Database): 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• Original Data Provider (Database): Japan Link Center; https://japanlinkcenter.org/top; Crossref; https://www.crossref.org; PubMed; https://pubmed.ncbi.nlm.nih.gov

• Summary, etc.: <p>Cardiomyocyte lipotoxicity and ferroptosis are the key to the development of diabetic cardiomyopathy (DCM). Perilipin 5 (PLIN5) is perceived as a significant target of DCM. This study aimed to focus on the role and mechanism of PLIN5 on lipotoxicity and ferroptosis in DCM.</p><p>Following transfection, mouse cardiomyocytes HL-1 were induced by 0.1 mM palmitic acid (PA) to set up lipotoxic cardiomyocyte models. The cell viability and lipid accumulation were evaluated by cell counting kit-8 assay and Oil red O staining, respectively. Ferrous ion (Fe²⁺), glutathione (GSH), malondialdehyde (MDA), and reactive oxygen species (ROS) levels were determined to verify the effects of PLIN5 or Pirin (PIR) on ferroptosis. Quantitative real-time reverse transcription polymerase chain reaction or Western blot was performed for quantitative analysis.</p><p>PLIN5 overexpression promoted the viability, GSH level, and expression of GPX4/PIR/intracellular P65, yet suppressed lipid accumulation, level of Fe²⁺/MDA/ROS, and expression of interleukin (IL)-1β/IL-18/intranuclear P65 in PA-stimulated HL-1 cells. PIR silencing counteracted the roles of PLIN5 overexpression in PA-stimulated HL-1 cells.</p><p>PLIN5 suppresses lipotoxicity and ferroptosis in cardiomyocyte via modulating PIR/NF-κB axis, hinting its potential as a therapeutic target in DCM.</p>
• DOI: 10.1536/ihj.24-002
• Access Restrictions: インターネット公開
• Data Provider (Database): 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp