Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway

• 資料種別: 文書・図像類
• タイトル: Temperature-sensitive defects of the GSP1gene, yeast Ran homologue, activate the Tel1-dependent pathway
• 著者・編者: HAYASHI, Naoyuki; MURAKAMI, Seishi; TSURUSAKI, Susumu; NAGAURA, Zen-ichiro; OKI, Masaya; NISHITANI, Hideo; KOBAYASHI, Masahiko; SHIMIZU, Hiroko; YAMAMOTO, Kenichi; NISHIMOTO, Takeharu
• 著者標目: HAYASHI, Naoyuki; MURAKAMI, Seishi; TSURUSAKI, Susumu; NAGAURA, Zen-ichiro; OKI, Masaya; NISHITANI, Hideo; KOBAYASHI, Masahiko; SHIMIZU, Hiroko; YAMAMOTO, Kenichi; NISHIMOTO, Takeharu
• 出版事項: Elsevier Science B.V.
• 出版年月日等: 2007-02
• 出版年（W3CDTF）: 2007-02
• タイトル（掲載誌）: Biochemical and Biophysical Research Communications
• 巻号年月日等（掲載誌）: 353 2
• 掲載巻: 353
• 掲載号: 2
• 掲載ページ: 330-336
• ISSN（掲載誌）: ISSN : 0006-291X
• 本文の言語コード: eng
• 件名標目: Ran; Gsp1; checkpoint; ATM kinase; Mec1; Tel1; Rad53; yeast; replication; signal transduction
• 対象利用者: 一般
• 一般注記: 出版タイプ： AO; RanGTPase is involved in many cellular processes. It functions in nuclear-cytosolic transport and centrosome formation. Ran also localizes to chromatin as RCC1 does, its guanine nucleotide exchange factor, but Ran's function on chromatin is not known. We found that gsp1, a temperature-sensitive mutant of GSP1, a Saccharomyces cerevisiae Ran homologue, suppressed the hydroxyurea (HU) and ultra violet (UV) sensitivities of the mec1 mutant. In UV-irradiated mec1 gsp1 cells, Rad53 was phosphorylated despite the lack of Mec1. This suppression depended on the TEL1 gene, given that the triple mutant, mec1 gsp1 tel1, was unable to grow. The gsp1 mutations also suppressed the HU sensitivity of the rad9 mutant in a Tel1-dependent manner, but not the HU sensitivity of the rad53 mutant. These results indicated that Rad53 was activated by the Tel1 pathway in mec1 gsp1 cells, suggesting that Gsp1 helps regulate the role switching the ATM family kinases Mec1 and Tel1.
• 関連情報（DOI）: 10.1016/j.bbrc.2006.12.028; https://doi.org/10.1016/j.bbrc.2006.12.028
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 福井大学 : 福井大学学術機関リポジトリ