MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells

• 資料種別: 博士論文
• タイトル: MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells
• 著者・編者: Iwawaki, Yuki; Mizusawa, Noriko; Iwata, Takeo; Higaki, Nobuaki; Goto, Takaharu; Watanabe, Megumi; Tomotake, Yoritoki; Ichikawa, Tetsuo; Yoshimoto, Katsuhiko
• 著者標目: Iwawaki, Yuki; Mizusawa, Noriko; Iwata, Takeo; Higaki, Nobuaki; Goto, Takaharu; Watanabe, Megumi; Tomotake, Yoritoki; Ichikawa, Tetsuo; Yoshimoto, Katsuhiko
• 出版事項: The Society for Biotechnology, Japan|Elsevier
• 出版年月日等: 2015-03-17
• 出版年（W3CDTF）: 2015-03-17
• 並列タイトル等: MC3T3-E1細胞における圧縮応力誘導性miR494-3pによる細胞増殖の阻害; FUNCTION OF miR-494-3p IN OSTEOBLASTS
• タイトル（掲載誌）: Journal of Bioscience and Bioengineering
• 巻号年月日等（掲載誌）: 120 4
• 掲載巻: 120
• 掲載号: 4
• 掲載ページ: 456-462
• ISSN（掲載誌）: ISSN : 1347-4421; ISSN : 1389-1723
• 授与機関名: 徳島大学
• 授与年月日: 2015-06-11
• 報告番号: 甲第2858号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: Mechanical stimuli; Compressive force; MicroRNA; Osteoblasts; Cell proliferation
• 対象利用者: 一般
• 一般注記: Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3'-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation in osteoblasts by up-regulating miR-494-3p followed by FGFR2 and ROCK1 gene repressions.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
• 関連情報（DOI）: 10.1016/j.jbiosc.2015.02.006
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/10/109479/20170929143537616147/LID201507171004.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109479/20170929143538251102/LID201507171005.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109479/20190822153655371185/k2858_fulltext.pdf fulltext