MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells

• Material Type: 博士論文
• Title: MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells
• Author/Editor: Iwawaki, Yuki; Mizusawa, Noriko; Iwata, Takeo; Higaki, Nobuaki; Goto, Takaharu; Watanabe, Megumi; Tomotake, Yoritoki; Ichikawa, Tetsuo; Yoshimoto, Katsuhiko
• Author Heading: Iwawaki, Yuki; Mizusawa, Noriko; Iwata, Takeo; Higaki, Nobuaki; Goto, Takaharu; Watanabe, Megumi; Tomotake, Yoritoki; Ichikawa, Tetsuo; Yoshimoto, Katsuhiko
• Publication, Distribution, etc.: The Society for Biotechnology, Japan|Elsevier
• Publication Date: 2015-03-17
• Publication Date (W3CDTF): 2015-03-17
• Alternative Title: MC3T3-E1細胞における圧縮応力誘導性miR494-3pによる細胞増殖の阻害; FUNCTION OF miR-494-3p IN OSTEOBLASTS
• Periodical title: Journal of Bioscience and Bioengineering
• No. or year of volume/issue: 120 4
• Volume: 120
• Issue: 4
• Pages: 456-462
• ISSN (Periodical Title): ISSN : 1347-4421; ISSN : 1389-1723
• Degree grantor/type: 徳島大学
• Date Granted: 2015-06-11
• Dissertation Number: 甲第2858号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Mechanical stimuli; Compressive force; MicroRNA; Osteoblasts; Cell proliferation
• Target Audience: 一般
• Note (General): Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3'-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation in osteoblasts by up-regulating miR-494-3p followed by FGFR2 and ROCK1 gene repressions.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
• Related Material (DOI): 10.1016/j.jbiosc.2015.02.006
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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