Histone Demethylase Jmjd3 Regulates Osteoblast Differentiation via Transcription Factors Runx2 and Osterix

• 資料種別: 博士論文
• タイトル: Histone Demethylase Jmjd3 Regulates Osteoblast Differentiation via Transcription Factors Runx2 and Osterix
• 著者・編者: Yang, Di; Okamura, Hirohiko; Nakashima, Yoshiki; Haneji, Tatsuji
• 著者標目: Yang, Di; Okamura, Hirohiko; Nakashima, Yoshiki; Haneji, Tatsuji
• 出版年月日等: 2013-10-08
• 出版年（W3CDTF）: 2013-10-08
• 並列タイトル等: ヒストンデメチラーゼJmjd3は転写因子Runx2とOsterixを介して骨芽細胞の分化を調節する; Regulation of osteoblast differentiation by Jmjd3
• タイトル（掲載誌）: The Journal of Biological Chemistry
• 巻号年月日等（掲載誌）: 288 47
• 掲載巻: 288
• 掲載号: 47
• 掲載ページ: 33530-33541
• 授与機関名: 徳島大学
• 授与年月日: 2015-09-16
• 報告番号: 甲第2862号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: Jmjd3; H3K27me3; BMP-2; Osteoblast differentiation; Histone demethylation; Runx2; Osterix
• 対象利用者: 一般
• 一般注記: Post-translational modifications of histones including methylation play important roles in cell differentiation. Jumonji domain-containing 3 (Jmjd3) is a histone demethylase, which specifically catalyzes the removal of trimethylation of histone H3 at lysine 27 (H3K27me3). In this study, we examined the expression of Jmjd3 in osteoblasts and its roles in osteoblast differentiation. Jmjd3 expression in the nucleus was induced in response to the stimulation of osteoblast differentiation as well as treatment of bone morphogenetic protein-2 (BMP-2). Either treatment with Noggin, an inhibitor of BMP-2, or silencing of Smad1/5, suppressed Jmjd3 expression during osteoblast differentiation. Silencing of Jmjd3 expression suppressed osteoblast differentiation through the expression of bone-related genes including Runx2, Osterix, Osteopontin, Bone sialoprotein (BSP), and Osteocalcin (OCN). Silencing of Jmjd3 decreased the promoter activities of Runx2 and Osterix and increased the level of H3K27me3 on the promoter regions of Runx2 and Osterix. Introduction of the exogenous Runx2 and Osterix partly rescued osteoblast differentiation in the shJmjd3 cells. The present results indicate that Jmjd3 plays important roles in osteoblast differentiation and regulates the expressions of BSP and OCN via transcription factors Runx2 and Osterix.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: Copyright : The American Society for Biochemistry and Molecular Biology.
• 関連情報（DOI）: 10.1074/jbc.M113.497040
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/10/109488/20170929143444447097/LID201509181013.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109488/20170929143444789508/LID201509181014.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109488/20170929143445128614/LID201509181015.pdf fulltext