Histone Demethylase Jmjd3 Regulates Osteoblast Differentiation via Transcription Factors Runx2 and Osterix

• Material Type: 博士論文
• Title: Histone Demethylase Jmjd3 Regulates Osteoblast Differentiation via Transcription Factors Runx2 and Osterix
• Author/Editor: Yang, Di; Okamura, Hirohiko; Nakashima, Yoshiki; Haneji, Tatsuji
• Author Heading: Yang, Di; Okamura, Hirohiko; Nakashima, Yoshiki; Haneji, Tatsuji
• Publication Date: 2013-10-08
• Publication Date (W3CDTF): 2013-10-08
• Alternative Title: ヒストンデメチラーゼJmjd3は転写因子Runx2とOsterixを介して骨芽細胞の分化を調節する; Regulation of osteoblast differentiation by Jmjd3
• Periodical title: The Journal of Biological Chemistry
• No. or year of volume/issue: 288 47
• Volume: 288
• Issue: 47
• Pages: 33530-33541
• Degree grantor/type: 徳島大学
• Date Granted: 2015-09-16
• Dissertation Number: 甲第2862号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Jmjd3; H3K27me3; BMP-2; Osteoblast differentiation; Histone demethylation; Runx2; Osterix
• Target Audience: 一般
• Note (General): Post-translational modifications of histones including methylation play important roles in cell differentiation. Jumonji domain-containing 3 (Jmjd3) is a histone demethylase, which specifically catalyzes the removal of trimethylation of histone H3 at lysine 27 (H3K27me3). In this study, we examined the expression of Jmjd3 in osteoblasts and its roles in osteoblast differentiation. Jmjd3 expression in the nucleus was induced in response to the stimulation of osteoblast differentiation as well as treatment of bone morphogenetic protein-2 (BMP-2). Either treatment with Noggin, an inhibitor of BMP-2, or silencing of Smad1/5, suppressed Jmjd3 expression during osteoblast differentiation. Silencing of Jmjd3 expression suppressed osteoblast differentiation through the expression of bone-related genes including Runx2, Osterix, Osteopontin, Bone sialoprotein (BSP), and Osteocalcin (OCN). Silencing of Jmjd3 decreased the promoter activities of Runx2 and Osterix and increased the level of H3K27me3 on the promoter regions of Runx2 and Osterix. Introduction of the exogenous Runx2 and Osterix partly rescued osteoblast differentiation in the shJmjd3 cells. The present results indicate that Jmjd3 plays important roles in osteoblast differentiation and regulates the expressions of BSP and OCN via transcription factors Runx2 and Osterix.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): Copyright : The American Society for Biochemistry and Molecular Biology.
• Related Material (DOI): 10.1074/jbc.M113.497040
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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