DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes

• 資料種別: 博士論文
• タイトル: DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes
• 著者・編者: Kuroda, Masashi; Tominaga, Ayako; Nakagawa, Kasumi; Nishiguchi, Misa; Sebe, Mayu; Miyatake, Yumiko; Kitamura, Tadahiro; Tsutsumi, Rie; Harada, Nagakatsu; Nakaya, Yutaka; Sakaue, Hiroshi
• 著者標目: Kuroda, Masashi; Tominaga, Ayako; Nakagawa, Kasumi; Nishiguchi, Misa; Sebe, Mayu; Miyatake, Yumiko; Kitamura, Tadahiro; Tsutsumi, Rie; Harada, Nagakatsu; Nakaya, Yutaka; Sakaue, Hiroshi
• 出版事項: PLOS
• 出版年月日等: 2016-08-05
• 出版年（W3CDTF）: 2016-08-05
• 並列タイトル等: DNAメチル化は3T3-L1脂肪細胞におけるレプチン発現を抑制する; DNA Methylation Suppresses Leptin Expression in Adipocytes
• タイトル（掲載誌）: PLOS ONE
• 巻号年月日等（掲載誌）: 11 8
• 掲載巻: 11
• 掲載号: 8
• 掲載ページ: e0160532-
• ISSN（掲載誌）: ISSN : 1932-6203
• 授与機関名: 徳島大学
• 授与年月日: 2017-03-23
• 報告番号: 甲第3087号
• 学位: 博士（栄養学）
• 本文の言語コード: eng
• 件名標目: Adipocyte; Leptin; DNA methylation; 3T3-L1; obesity
• 対象利用者: 一般
• 一般注記: Leptin is a key regulator of energy intake and expenditure. This peptide hormone is expressed in mouse white adipose tissue, but hardly expressed in 3T3-L1 adipocytes. Using bisulfite sequencing, we found that CpG islands in the leptin promoter are highly methylated in 3T3-L1cells. 5-azacytidine, an inhibitor of DNA methyltransferase, markedly increased leptin expression as pre-adipocytes matured into adipocytes. Remarkably, leptin expression was stimulated by insulin in adipocytes derived from precursor cells exposed to 5-azacytidine, but suppressed by thiazolidinedione and dexamethasone. In contrast, adipocytes derived from untreated precursor cells were unresponsive to both 5-azacytidine and hormonal stimuli, although lipid accumulation was sufficient to boost leptin expression in the absence of demethylation. Taken together, the results suggest that leptin expression in 3T3-L1 cells requires DNA demethylation prior to adipogenesis, transcriptional activation during adipogenesis, and lipid accumulation after adipogenesis.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This is an open access article distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• 関連情報（DOI）: 10.1371/journal.pone.0160532
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/110107/2017092914230811788/LID201704261022.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/110107/20170929142308314527/LID201704261023.pdf fulltext