DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes

• Material Type: 博士論文
• Title: DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes
• Author/Editor: Kuroda, Masashi; Tominaga, Ayako; Nakagawa, Kasumi; Nishiguchi, Misa; Sebe, Mayu; Miyatake, Yumiko; Kitamura, Tadahiro; Tsutsumi, Rie; Harada, Nagakatsu; Nakaya, Yutaka; Sakaue, Hiroshi
• Author Heading: Kuroda, Masashi; Tominaga, Ayako; Nakagawa, Kasumi; Nishiguchi, Misa; Sebe, Mayu; Miyatake, Yumiko; Kitamura, Tadahiro; Tsutsumi, Rie; Harada, Nagakatsu; Nakaya, Yutaka; Sakaue, Hiroshi
• Publication, Distribution, etc.: PLOS
• Publication Date: 2016-08-05
• Publication Date (W3CDTF): 2016-08-05
• Alternative Title: DNAメチル化は3T3-L1脂肪細胞におけるレプチン発現を抑制する; DNA Methylation Suppresses Leptin Expression in Adipocytes
• Periodical title: PLOS ONE
• No. or year of volume/issue: 11 8
• Volume: 11
• Issue: 8
• Pages: e0160532-
• ISSN (Periodical Title): ISSN : 1932-6203
• Degree grantor/type: 徳島大学
• Date Granted: 2017-03-23
• Dissertation Number: 甲第3087号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: Adipocyte; Leptin; DNA methylation; 3T3-L1; obesity
• Target Audience: 一般
• Note (General): Leptin is a key regulator of energy intake and expenditure. This peptide hormone is expressed in mouse white adipose tissue, but hardly expressed in 3T3-L1 adipocytes. Using bisulfite sequencing, we found that CpG islands in the leptin promoter are highly methylated in 3T3-L1cells. 5-azacytidine, an inhibitor of DNA methyltransferase, markedly increased leptin expression as pre-adipocytes matured into adipocytes. Remarkably, leptin expression was stimulated by insulin in adipocytes derived from precursor cells exposed to 5-azacytidine, but suppressed by thiazolidinedione and dexamethasone. In contrast, adipocytes derived from untreated precursor cells were unresponsive to both 5-azacytidine and hormonal stimuli, although lipid accumulation was sufficient to boost leptin expression in the absence of demethylation. Taken together, the results suggest that leptin expression in 3T3-L1 cells requires DNA demethylation prior to adipogenesis, transcriptional activation during adipogenesis, and lipid accumulation after adipogenesis.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
• Related Material (DOI): 10.1371/journal.pone.0160532
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/110107/2017092914230811788/LID201704261022.pdf fulltext

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