Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells

• 資料種別: 博士論文
• タイトル: Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells
• 著者・編者: Shiozaki, Yuji; Segawa, Hiroko; Ohnishi, Saori; Ohi, Akiko; Ito, Mikiko; Kaneko, Ichiro; Kido, Shinsuke; Tatsumi, Sawako; Miyamoto, Ken-ichi
• 著者標目: Shiozaki, Yuji; Segawa, Hiroko; Ohnishi, Saori; Ohi, Akiko; Ito, Mikiko; Kaneko, Ichiro; Kido, Shinsuke; Tatsumi, Sawako; Miyamoto, Ken-ichi
• 出版事項: Faculty of Medicine Tokushima University
• 出版年月日等: 2015-08
• 出版年（W3CDTF）: 2015-08
• 並列タイトル等: フクロネズミ腎近位尿細管細胞におけるナトリウム依存性リン酸トランスポーターNaPi-IIcと細胞内vacuole形成の関連; NaPi-IIc induces vacuole formation in OK cells
• タイトル（掲載誌）: The Journal of Medical Investigation
• 巻号年月日等（掲載誌）: 62 3-4
• 掲載巻: 62
• 掲載号: 3-4
• 掲載ページ: 209-218
• ISSN（掲載誌）: ISSN : 1349-6867; ISSN : 1343-1420
• 授与機関名: 徳島大学
• 授与年月日: 2015-07-23
• 報告番号: 甲第2867号
• 学位: 博士（栄養学）
• 本文の言語コード: eng
• 件名標目: proximal tubule; SLC34A3; opossum kidney cells; proliferation; vacuole
• 対象利用者: 一般
• 一般注記: NaPi-IIc/SLC34A3 is a sodium-dependent inorganic phosphate (Pi) transporter in the renal proximal tubules and its mutations cause hereditary hypophosphatemic rickets with hypercalciuria (HHRH). In the present study, we created a specific antibody for opossum SLC34A3, NaPi-IIc (oNaPi-IIc), and analyzed its localization and regulation in opossum kidney cells (a tissue culture model of proximal tubular cells). Immunoreactive oNaPi-IIc protein levels increased during the proliferative phase and decreased during differentiation. Moreover, stimulating cell growth upregulated oNaPi-IIc protein levels, whereas suppressing cell proliferation downregulated oNaPi-IIc protein levels. Immunocytochemistry revealed that endogenous and exogenous oNaPi-IIc proteins localized at the protrusion of the plasma membrane, which is a phosphatidylinositol 4,5-bisphosphate (PIP2) rich-membrane, and at the intracellular vacuolar membrane. Exogenous NaPi-IIc also induced cellular vacuoles and localized in the plasma membrane. The ability to form vacuoles is specific to electroneutral NaPi-IIc, and not electrogenic NaPi-IIa or NaPi-IIb. In addition, mutations of NaPi-IIc (S138F and R468W) in HHRH did not cause cellular PIP2-rich vacuoles. In conclusion, our data anticipate that NaPi-IIc may regulate PIP2 production at the plasma membrane and cellular vesicle formation.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.2152/jmi.62.209
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/111281/20190802141435843091/LID201508051001.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/111281/20190802141436226807/LID201509301001.pdf fulltext