Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells

• Material Type: 博士論文
• Title: Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells
• Author/Editor: Shiozaki, Yuji; Segawa, Hiroko; Ohnishi, Saori; Ohi, Akiko; Ito, Mikiko; Kaneko, Ichiro; Kido, Shinsuke; Tatsumi, Sawako; Miyamoto, Ken-ichi
• Author Heading: Shiozaki, Yuji; Segawa, Hiroko; Ohnishi, Saori; Ohi, Akiko; Ito, Mikiko; Kaneko, Ichiro; Kido, Shinsuke; Tatsumi, Sawako; Miyamoto, Ken-ichi
• Publication, Distribution, etc.: Faculty of Medicine Tokushima University
• Publication Date: 2015-08
• Publication Date (W3CDTF): 2015-08
• Alternative Title: フクロネズミ腎近位尿細管細胞におけるナトリウム依存性リン酸トランスポーターNaPi-IIcと細胞内vacuole形成の関連; NaPi-IIc induces vacuole formation in OK cells
• Periodical title: The Journal of Medical Investigation
• No. or year of volume/issue: 62 3-4
• Volume: 62
• Issue: 3-4
• Pages: 209-218
• ISSN (Periodical Title): ISSN : 1349-6867; ISSN : 1343-1420
• Degree grantor/type: 徳島大学
• Date Granted: 2015-07-23
• Dissertation Number: 甲第2867号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: proximal tubule; SLC34A3; opossum kidney cells; proliferation; vacuole
• Target Audience: 一般
• Note (General): NaPi-IIc/SLC34A3 is a sodium-dependent inorganic phosphate (Pi) transporter in the renal proximal tubules and its mutations cause hereditary hypophosphatemic rickets with hypercalciuria (HHRH). In the present study, we created a specific antibody for opossum SLC34A3, NaPi-IIc (oNaPi-IIc), and analyzed its localization and regulation in opossum kidney cells (a tissue culture model of proximal tubular cells). Immunoreactive oNaPi-IIc protein levels increased during the proliferative phase and decreased during differentiation. Moreover, stimulating cell growth upregulated oNaPi-IIc protein levels, whereas suppressing cell proliferation downregulated oNaPi-IIc protein levels. Immunocytochemistry revealed that endogenous and exogenous oNaPi-IIc proteins localized at the protrusion of the plasma membrane, which is a phosphatidylinositol 4,5-bisphosphate (PIP2) rich-membrane, and at the intracellular vacuolar membrane. Exogenous NaPi-IIc also induced cellular vacuoles and localized in the plasma membrane. The ability to form vacuoles is specific to electroneutral NaPi-IIc, and not electrogenic NaPi-IIa or NaPi-IIb. In addition, mutations of NaPi-IIc (S138F and R468W) in HHRH did not cause cellular PIP2-rich vacuoles. In conclusion, our data anticipate that NaPi-IIc may regulate PIP2 production at the plasma membrane and cellular vesicle formation.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2152/jmi.62.209
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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