Establishment and characterization of a clear cell odontogenic carcinoma cell line with EWSR1-ATF1 fusion gene

• 資料種別: 博士論文
• タイトル: Establishment and characterization of a clear cell odontogenic carcinoma cell line with EWSR1-ATF1 fusion gene
• 著者・編者: Kujiraoka, Satoko; Tsunematsu, Takaaki; Sato, Yukiko; Yoshida, Maki; Ishikawa, Ayataka; Tohyama, Rei; Tanaka, Michio; Kobayashi, Yutaka; Kondo, Tomoyuki; Ushio, Aya; Otsuka, Kunihiro; Kurosawa, Mie; Saito, Masako; Yamada, Akiko; Arakaki, Rieko; Nagai, Hirokazu; Nikai, Hiromasa; Takeuchi, Kengo; Nagao, Toshitaka; Miyamoto, Youji; Ishimaru, Naozumi; Kudo, Yasusei
• 著者標目: Kujiraoka, Satoko; Tsunematsu, Takaaki; Sato, Yukiko; Yoshida, Maki; Ishikawa, Ayataka; Tohyama, Rei; Tanaka, Michio; Kobayashi, Yutaka; Kondo, Tomoyuki; Ushio, Aya; Otsuka, Kunihiro; Kurosawa, Mie; Saito, Masako; Yamada, Akiko; Arakaki, Rieko; Nagai, Hirokazu; Nikai, Hiromasa; Takeuchi, Kengo; Nagao, Toshitaka; Miyamoto, Youji; Ishimaru, Naozumi; Kudo, Yasusei
• 出版事項: Elsevier
• 出版年月日等: 2017-04-10
• 出版年（W3CDTF）: 2017-04-10
• 並列タイトル等: EWSR1-ATF1融合遺伝子を持つ歯原性明細胞癌細胞株の樹立と性状解析
• タイトル（掲載誌）: Oral Oncology
• 巻号年月日等（掲載誌）: 69
• 掲載巻: 69
• 掲載ページ: 46-55
• ISSN（掲載誌）: ISSN : 1368-8375
• 授与機関名: 徳島大学
• 授与年月日: 2018-03-23
• 報告番号: 甲第3177号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: Clear cell odontogenic carcinoma; Malignant odontogenic tumor; Hyalinizing clear cell carcinoma; EWSR1-ATF1 fusion genes
• 対象利用者: 一般
• 一般注記: Objective: Clear cell odontogenic carcinoma (CCOC) is a rare malignant odontogenic tumor (MOT) characterized by sheets and lobules of vacuolated and clear cells. To understand the biology of CCOC, we established a new cell line, CCOC-T, with EWSR1-ATF1 fusion gene from a mandible tumor with distant metastasis and characterized this cell line. Materials and methods: To detect the EWSR1-ATF1 fusion gene, we used three CCOC cases, including the present case, by RT-PCR and FISH analysis. We characterized established CCOC-T cells by checking cell growth, invasion and the expression of odontogenic factors and bone-related factors. Moreover, the gene expression profile of CCOC-T cells was examined by microarray analysis. Results: Histologically, the primary tumor was comprised of cords and nests containing clear and squamoid cells separated by fibrous septa. In addition, ameloblastomatous islands with palisaded peripheral cells were observed, indicating probable odontogenic origin. This tumor expressed the fusion gene EWSR1-ATF1, which underlies the etiology of hyalinizing clear cell carcinoma (HCCC) and potentially that of CCOC. We found a breakpoint in the EWSR1-ATF1 fusion to be the same as that reported in HCCC. Established CCOC-T cells grew extremely slowly, but the cells showed highly invasive activity. Moreover, CCOC-T cells expressed bone-related molecules, odontogenic factors, and epithelial mesenchymal transition (EMT)-related molecules. Conclusion: To the best of our knowledge, this is the first report on the establishment of a CCOC cell line. CCOC-T cells serve as a useful in vitro model for understanding the pathogenesis and nature of MOT.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1016/j.oraloncology.2017.04.003
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/111726/20180607151456506281/k3177_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/111726/2018060715145779998/k3177_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/111726/20180607151457544271/k3177_fulltext.pdf fulltext