Establishment and characterization of a clear cell odontogenic carcinoma cell line with EWSR1-ATF1 fusion gene

• Material Type: 博士論文
• Title: Establishment and characterization of a clear cell odontogenic carcinoma cell line with EWSR1-ATF1 fusion gene
• Author/Editor: Kujiraoka, Satoko; Tsunematsu, Takaaki; Sato, Yukiko; Yoshida, Maki; Ishikawa, Ayataka; Tohyama, Rei; Tanaka, Michio; Kobayashi, Yutaka; Kondo, Tomoyuki; Ushio, Aya; Otsuka, Kunihiro; Kurosawa, Mie; Saito, Masako; Yamada, Akiko; Arakaki, Rieko; Nagai, Hirokazu; Nikai, Hiromasa; Takeuchi, Kengo; Nagao, Toshitaka; Miyamoto, Youji; Ishimaru, Naozumi; Kudo, Yasusei
• Author Heading: Kujiraoka, Satoko; Tsunematsu, Takaaki; Sato, Yukiko; Yoshida, Maki; Ishikawa, Ayataka; Tohyama, Rei; Tanaka, Michio; Kobayashi, Yutaka; Kondo, Tomoyuki; Ushio, Aya; Otsuka, Kunihiro; Kurosawa, Mie; Saito, Masako; Yamada, Akiko; Arakaki, Rieko; Nagai, Hirokazu; Nikai, Hiromasa; Takeuchi, Kengo; Nagao, Toshitaka; Miyamoto, Youji; Ishimaru, Naozumi; Kudo, Yasusei
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2017-04-10
• Publication Date (W3CDTF): 2017-04-10
• Alternative Title: EWSR1-ATF1融合遺伝子を持つ歯原性明細胞癌細胞株の樹立と性状解析
• Periodical title: Oral Oncology
• No. or year of volume/issue: 69
• Volume: 69
• Pages: 46-55
• ISSN (Periodical Title): ISSN : 1368-8375
• Degree grantor/type: 徳島大学
• Date Granted: 2018-03-23
• Dissertation Number: 甲第3177号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Clear cell odontogenic carcinoma; Malignant odontogenic tumor; Hyalinizing clear cell carcinoma; EWSR1-ATF1 fusion genes
• Target Audience: 一般
• Note (General): Objective: Clear cell odontogenic carcinoma (CCOC) is a rare malignant odontogenic tumor (MOT) characterized by sheets and lobules of vacuolated and clear cells. To understand the biology of CCOC, we established a new cell line, CCOC-T, with EWSR1-ATF1 fusion gene from a mandible tumor with distant metastasis and characterized this cell line. Materials and methods: To detect the EWSR1-ATF1 fusion gene, we used three CCOC cases, including the present case, by RT-PCR and FISH analysis. We characterized established CCOC-T cells by checking cell growth, invasion and the expression of odontogenic factors and bone-related factors. Moreover, the gene expression profile of CCOC-T cells was examined by microarray analysis. Results: Histologically, the primary tumor was comprised of cords and nests containing clear and squamoid cells separated by fibrous septa. In addition, ameloblastomatous islands with palisaded peripheral cells were observed, indicating probable odontogenic origin. This tumor expressed the fusion gene EWSR1-ATF1, which underlies the etiology of hyalinizing clear cell carcinoma (HCCC) and potentially that of CCOC. We found a breakpoint in the EWSR1-ATF1 fusion to be the same as that reported in HCCC. Established CCOC-T cells grew extremely slowly, but the cells showed highly invasive activity. Moreover, CCOC-T cells expressed bone-related molecules, odontogenic factors, and epithelial mesenchymal transition (EMT)-related molecules. Conclusion: To the best of our knowledge, this is the first report on the establishment of a CCOC cell line. CCOC-T cells serve as a useful in vitro model for understanding the pathogenesis and nature of MOT.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.oraloncology.2017.04.003
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/111726/20180607151456506281/k3177_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/111726/2018060715145779998/k3177_review.pdf fulltext

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