Expression of cytokine-induced neutrophil chemoattractant suppresses tumor necrosis factor alpha expression and thereby prevents the follicles from undergoing atresia and apoptosis

• 資料種別: 博士論文
• タイトル: Expression of cytokine-induced neutrophil chemoattractant suppresses tumor necrosis factor alpha expression and thereby prevents the follicles from undergoing atresia and apoptosis
• 著者・編者: Tanaka, Yu; Kuwahara, Akira; Ushigoe, Kenjiro; Yano, Yuya; Taniguchi, Yuka; Yamamoto, Yuri; Matsuzaki, Toshiya; Yasui, Toshiyuki; Irahara, Minoru
• 著者標目: Tanaka, Yu; Kuwahara, Akira; Ushigoe, Kenjiro; Yano, Yuya; Taniguchi, Yuka; Yamamoto, Yuri; Matsuzaki, Toshiya; Yasui, Toshiyuki; Irahara, Minoru
• 出版事項: Wiley|Japan Society for Reproductive Medicine
• 出版年月日等: 2017-03-12
• 出版年（W3CDTF）: 2017-03-12
• 並列タイトル等: サイトカイン誘発好中球化学誘引物質の発現はTNFα発現を抑制することにより卵胞の閉鎖およびアポトーシスから回避させる
• タイトル（掲載誌）: Reproductive Medicine and Biology
• 巻号年月日等（掲載誌）: 16 2
• 掲載巻: 16
• 掲載号: 2
• 掲載ページ: 157-165
• ISSN（掲載誌）: ISSN : 1447-0578
• 授与機関名: 徳島大学
• 授与年月日: 2019-03-19
• 報告番号: 乙第2107号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Apoptosis; follicle; ovary; ovulation; tumor necrosis factor alpha
• 対象利用者: 一般
• 一般注記: Aim: Cytokine-induced neutrophil chemoattractant (CINC/gro) is a CXC family chemokine, similar to interleukin-8 in rats, and is one of the factors that regulates ovulation. However, the mechanism that regulates atresia of the ovaries postovulation is not clearly defined. Methods: Whether antibody-blocking of CINC/gro can alter the number of ovulated oocytes and modulate neutrophil infiltration was investigated. The effect of the antibody on the level of inflammatory cytokine production and follicular atresia was examined. Apoptosis was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and via analysis of the messenger RNA expression of Bcl-2 and Bcl2-associated X (Bax). Results: The anti-CINC/gro antibody treatment decreased the number of ovulated oocytes. The messenger RNA levels of cyclooxygenase-2 and interleukin-1 beta were decreased by the antibody treatment, whereas that of tumor necrosis factor (TNF) alpha was increased. The TUNEL analysis revealed a larger number of apoptotic cells in the antibody group, compared with those in the control group, as well as a significant increase in the Bax/Bcl-2 ratio 24 hours after human chorionic gonadotropin administration. Conclusion: These findings suggest that ovulation is accelerated by neutrophil infiltration into the theca layer. The CINC/gro appears to synergize with interleukin-1 beta for ovulation. By contrast, the data suggest that CINC/gro expression suppresses TNF alpha expression and that CINC/gro expression therefore prevents the follicles from undergoing atresia and apoptosis.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This is an open access article under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2017 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.
• 関連情報（DOI）: 10.1002/rmb2.12022
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/113212/20190403112242374175/o2107_abstract_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/113212/20190403112242873347/o2107_fulltext.pdf fulltext