Expression of cytokine-induced neutrophil chemoattractant suppresses tumor necrosis factor alpha expression and thereby prevents the follicles from undergoing atresia and apoptosis

• Material Type: 博士論文
• Title: Expression of cytokine-induced neutrophil chemoattractant suppresses tumor necrosis factor alpha expression and thereby prevents the follicles from undergoing atresia and apoptosis
• Author/Editor: Tanaka, Yu; Kuwahara, Akira; Ushigoe, Kenjiro; Yano, Yuya; Taniguchi, Yuka; Yamamoto, Yuri; Matsuzaki, Toshiya; Yasui, Toshiyuki; Irahara, Minoru
• Author Heading: Tanaka, Yu; Kuwahara, Akira; Ushigoe, Kenjiro; Yano, Yuya; Taniguchi, Yuka; Yamamoto, Yuri; Matsuzaki, Toshiya; Yasui, Toshiyuki; Irahara, Minoru
• Publication, Distribution, etc.: Wiley|Japan Society for Reproductive Medicine
• Publication Date: 2017-03-12
• Publication Date (W3CDTF): 2017-03-12
• Alternative Title: サイトカイン誘発好中球化学誘引物質の発現はTNFα発現を抑制することにより卵胞の閉鎖およびアポトーシスから回避させる
• Periodical title: Reproductive Medicine and Biology
• No. or year of volume/issue: 16 2
• Volume: 16
• Issue: 2
• Pages: 157-165
• ISSN (Periodical Title): ISSN : 1447-0578
• Degree grantor/type: 徳島大学
• Date Granted: 2019-03-19
• Dissertation Number: 乙第2107号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Apoptosis; follicle; ovary; ovulation; tumor necrosis factor alpha
• Target Audience: 一般
• Note (General): Aim: Cytokine-induced neutrophil chemoattractant (CINC/gro) is a CXC family chemokine, similar to interleukin-8 in rats, and is one of the factors that regulates ovulation. However, the mechanism that regulates atresia of the ovaries postovulation is not clearly defined. Methods: Whether antibody-blocking of CINC/gro can alter the number of ovulated oocytes and modulate neutrophil infiltration was investigated. The effect of the antibody on the level of inflammatory cytokine production and follicular atresia was examined. Apoptosis was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and via analysis of the messenger RNA expression of Bcl-2 and Bcl2-associated X (Bax). Results: The anti-CINC/gro antibody treatment decreased the number of ovulated oocytes. The messenger RNA levels of cyclooxygenase-2 and interleukin-1 beta were decreased by the antibody treatment, whereas that of tumor necrosis factor (TNF) alpha was increased. The TUNEL analysis revealed a larger number of apoptotic cells in the antibody group, compared with those in the control group, as well as a significant increase in the Bax/Bcl-2 ratio 24 hours after human chorionic gonadotropin administration. Conclusion: These findings suggest that ovulation is accelerated by neutrophil infiltration into the theca layer. The CINC/gro appears to synergize with interleukin-1 beta for ovulation. By contrast, the data suggest that CINC/gro expression suppresses TNF alpha expression and that CINC/gro expression therefore prevents the follicles from undergoing atresia and apoptosis.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2017 The Authors. Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine.
• Related Material (DOI): 10.1002/rmb2.12022
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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