Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)

• 資料種別: 博士論文
• タイトル: Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)
• 著者・編者: Naritani, Mio; Inoue, Miho; Raju, Resmi; Miyagi, Mayu; Oshima, Masamitsu; Matsuka, Yoshizo
• 著者標目: Naritani, Mio; Inoue, Miho; Raju, Resmi; Miyagi, Mayu; Oshima, Masamitsu; Matsuka, Yoshizo
• 出版事項: THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
• 出版年月日等: 2019-04-24
• 出版年（W3CDTF）: 2019-04-24
• 並列タイトル等: TNF-α短期刺激による骨髄由来間葉系幹細胞の細胞動態解析; Effect of Short Term TNF-α Stimulation of BMSCs
• タイトル（掲載誌）: Journal of Hard Tissue Biology
• 巻号年月日等（掲載誌）: 28 2
• 掲載巻: 28
• 掲載号: 2
• 掲載ページ: 99-108
• ISSN（掲載誌）: ISSN : 1341-7649; ISSN : 1880-828X
• 授与機関名: 徳島大学
• 授与年月日: 2019-03-22
• 報告番号: 甲第3282号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: TNF-α; Bone marrow-derived mesenchymal stem cells; Short-term stimulation; Cell proliferation; Cell differentiation
• 対象利用者: 一般
• 一般注記: Bone marrow-derived mesenchymal stem cells (BMSCs) have considerable potential for self-renewal and multi-differentiation. Tumor necrosis factor-α (TNF-α) is an inflammatory cytokine and is involved in tissue regeneration during wound healing. It was already reported that cultured human dental pulp cells acquire stem cell properties following short-term stimulation by TNF-α. However, it has not been clarified if BMSCs acquire stem cell properties after TNF-α treatment. The purpose of this study was to investigate the effect of short-term stimulation with TNF-α on BMSCs. Rat BMSCs were cultured up to 60% confluence and then incubated with 1–100 ng/ml of recombinant rat TNF-α (rTNF-α) for a further 2 days. After reaching subconfluence, cells were passaged once to remove rTNF-α completely before subsequent assays. Cells in the control group were passaged without stimulation. Expression levels of Nanog and Oct4 stem cell markers were significantly increased in the rTNF-α 10 ng/ml stimulation group. rTNF-α stimulation did not affect cell proliferation compared with the control group. However, rTNF-α stimulation led to a delay in cell differentiation. This study suggests that short-term TNF-α stimulation of BMSCs significantly increased their stem cell phenotype, but delayed their osteogenic cell differentiation.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.2485/jhtb.28.99
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/113372/20190610110745783229/k3282_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/113372/20190610110746447998/k3282_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/113372/20190613154544983095/k3282_fulltext.pdf fulltext