Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)

• Material Type: 博士論文
• Title: Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)
• Author/Editor: Naritani, Mio; Inoue, Miho; Raju, Resmi; Miyagi, Mayu; Oshima, Masamitsu; Matsuka, Yoshizo
• Author Heading: Naritani, Mio; Inoue, Miho; Raju, Resmi; Miyagi, Mayu; Oshima, Masamitsu; Matsuka, Yoshizo
• Publication, Distribution, etc.: THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
• Publication Date: 2019-04-24
• Publication Date (W3CDTF): 2019-04-24
• Alternative Title: TNF-α短期刺激による骨髄由来間葉系幹細胞の細胞動態解析; Effect of Short Term TNF-α Stimulation of BMSCs
• Periodical title: Journal of Hard Tissue Biology
• No. or year of volume/issue: 28 2
• Volume: 28
• Issue: 2
• Pages: 99-108
• ISSN (Periodical Title): ISSN : 1341-7649; ISSN : 1880-828X
• Degree grantor/type: 徳島大学
• Date Granted: 2019-03-22
• Dissertation Number: 甲第3282号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: TNF-α; Bone marrow-derived mesenchymal stem cells; Short-term stimulation; Cell proliferation; Cell differentiation
• Target Audience: 一般
• Note (General): Bone marrow-derived mesenchymal stem cells (BMSCs) have considerable potential for self-renewal and multi-differentiation. Tumor necrosis factor-α (TNF-α) is an inflammatory cytokine and is involved in tissue regeneration during wound healing. It was already reported that cultured human dental pulp cells acquire stem cell properties following short-term stimulation by TNF-α. However, it has not been clarified if BMSCs acquire stem cell properties after TNF-α treatment. The purpose of this study was to investigate the effect of short-term stimulation with TNF-α on BMSCs. Rat BMSCs were cultured up to 60% confluence and then incubated with 1–100 ng/ml of recombinant rat TNF-α (rTNF-α) for a further 2 days. After reaching subconfluence, cells were passaged once to remove rTNF-α completely before subsequent assays. Cells in the control group were passaged without stimulation. Expression levels of Nanog and Oct4 stem cell markers were significantly increased in the rTNF-α 10 ng/ml stimulation group. rTNF-α stimulation did not affect cell proliferation compared with the control group. However, rTNF-α stimulation led to a delay in cell differentiation. This study suggests that short-term TNF-α stimulation of BMSCs significantly increased their stem cell phenotype, but delayed their osteogenic cell differentiation.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2485/jhtb.28.99
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

Table of Contents

http://repo.lib.tokushima-u.ac.jp/files/public/11/113372/20190610110745783229/k3282_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/113372/20190610110746447998/k3282_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/113372/20190613154544983095/k3282_fulltext.pdf fulltext