Epigallocatechin-3-gallate Enhances Radiation Sensitivity in Colorectal Cancer Cells Through Nrf2 Activation and Autophagy

• 資料種別: 博士論文
• タイトル: Epigallocatechin-3-gallate Enhances Radiation Sensitivity in Colorectal Cancer Cells Through Nrf2 Activation and Autophagy
• 著者・編者: Enkhbat, Tumenjin; Nishi, Masaaki; Yoshikawa, Kozo; Higashijima, Jun; Tokunaga, Takuya; Takasu, Chie; Kashihara, Hideya; Ishikawa, Daichi; Tominaga, Masahide; Shimada, Mitsuo
• 著者標目: Enkhbat, Tumenjin; Nishi, Masaaki; Yoshikawa, Kozo; Higashijima, Jun; Tokunaga, Takuya; Takasu, Chie; Kashihara, Hideya; Ishikawa, Daichi; Tominaga, Masahide; Shimada, Mitsuo
• 出版事項: The International Institute of Anticancer Research
• 出版年月日等: 2018-11-05
• 出版年（W3CDTF）: 2018-11-05
• 並列タイトル等: Epigallocatechin-3-gallateは大腸癌細胞株においてNrf2とオートファジーを介して放射線感受性を増強する; EGCG Enhances Radiation Sensitivity in Colorectal Cancer Cells
• タイトル（掲載誌）: Anticancer Research
• 巻号年月日等（掲載誌）: 38 11
• 掲載巻: 38
• 掲載号: 11
• 掲載ページ: 6247-6252
• ISSN（掲載誌）: ISSN : 0250-7005; ISSN : 1791-7530
• 授与機関名: 徳島大学
• 授与年月日: 2019-03-22
• 報告番号: 甲第3256号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Epigallocatechin-3-gallate; Radiation; Colorectal cancer; Nuclear factor (erythroid-derived 2)-like 2; Autophagy
• 対象利用者: 一般
• 一般注記: Background/Aim: Epigallocatechin-3-gallate (EGCG) is a major polyphenolic component of green tea. EGCG plays a potential role in radio-sensitizing cancer cells. The combined effect of EGCG and radiation was investigated in a colorectal cancer cell line, focusing on nuclear factor (erythroid-derived 2)-like 2 (Nrf2) autophagy signalling. Materials and Methods: HCT-116 cells were treated with 12.5 μM EGCG for different periods of time, 2 Gy radiation, or both. Cell viability was determined with the WST-8 assay. The number of colonies was determined with the colony formation assay. mRNA expression of LC3 and caspase-9 was analyzed with quantitative real-time polymerase chain reaction. Results: Combination treatment with EGCG and radiation significantly decreased the growth of HCT-116 cells. The number of colonies was reduced to 34.2% compared to the control group. Immunofluorescence microscopy images showed that nuclear translocation of Nrf2 was significantly increased when cells were treated with the combination of EGCG and radiation compared to the control and single-treatment groups. Combined treatment with EGCG and radiation significantly induced LC3 and caspase-9 mRNA expression. Conclusion: EGCG increased the sensitivity of colorectal cancer cells to radiation by inhibiting cell proliferation and inducing Nrf2 nuclear translocation and autophagy.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.21873/anticanres.12980
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/113340/2019061709132466680/k3256_abstract_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/113340/20210729121205902421/k3256_fulltext.pdf fulltext