Epigallocatechin-3-gallate Enhances Radiation Sensitivity in Colorectal Cancer Cells Through Nrf2 Activation and Autophagy

• Material Type: 博士論文
• Title: Epigallocatechin-3-gallate Enhances Radiation Sensitivity in Colorectal Cancer Cells Through Nrf2 Activation and Autophagy
• Author/Editor: Enkhbat, Tumenjin; Nishi, Masaaki; Yoshikawa, Kozo; Higashijima, Jun; Tokunaga, Takuya; Takasu, Chie; Kashihara, Hideya; Ishikawa, Daichi; Tominaga, Masahide; Shimada, Mitsuo
• Author Heading: Enkhbat, Tumenjin; Nishi, Masaaki; Yoshikawa, Kozo; Higashijima, Jun; Tokunaga, Takuya; Takasu, Chie; Kashihara, Hideya; Ishikawa, Daichi; Tominaga, Masahide; Shimada, Mitsuo
• Publication, Distribution, etc.: The International Institute of Anticancer Research
• Publication Date: 2018-11-05
• Publication Date (W3CDTF): 2018-11-05
• Alternative Title: Epigallocatechin-3-gallateは大腸癌細胞株においてNrf2とオートファジーを介して放射線感受性を増強する; EGCG Enhances Radiation Sensitivity in Colorectal Cancer Cells
• Periodical title: Anticancer Research
• No. or year of volume/issue: 38 11
• Volume: 38
• Issue: 11
• Pages: 6247-6252
• ISSN (Periodical Title): ISSN : 0250-7005; ISSN : 1791-7530
• Degree grantor/type: 徳島大学
• Date Granted: 2019-03-22
• Dissertation Number: 甲第3256号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Epigallocatechin-3-gallate; Radiation; Colorectal cancer; Nuclear factor (erythroid-derived 2)-like 2; Autophagy
• Target Audience: 一般
• Note (General): Background/Aim: Epigallocatechin-3-gallate (EGCG) is a major polyphenolic component of green tea. EGCG plays a potential role in radio-sensitizing cancer cells. The combined effect of EGCG and radiation was investigated in a colorectal cancer cell line, focusing on nuclear factor (erythroid-derived 2)-like 2 (Nrf2) autophagy signalling. Materials and Methods: HCT-116 cells were treated with 12.5 μM EGCG for different periods of time, 2 Gy radiation, or both. Cell viability was determined with the WST-8 assay. The number of colonies was determined with the colony formation assay. mRNA expression of LC3 and caspase-9 was analyzed with quantitative real-time polymerase chain reaction. Results: Combination treatment with EGCG and radiation significantly decreased the growth of HCT-116 cells. The number of colonies was reduced to 34.2% compared to the control group. Immunofluorescence microscopy images showed that nuclear translocation of Nrf2 was significantly increased when cells were treated with the combination of EGCG and radiation compared to the control and single-treatment groups. Combined treatment with EGCG and radiation significantly induced LC3 and caspase-9 mRNA expression. Conclusion: EGCG increased the sensitivity of colorectal cancer cells to radiation by inhibiting cell proliferation and inducing Nrf2 nuclear translocation and autophagy.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.21873/anticanres.12980
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/113340/2019061709132466680/k3256_abstract_review.pdf fulltext

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