Blue light-emitting diodes induce autophagy in colon cancer cells by Opsin 3

• 資料種別: 博士論文
• タイトル: Blue light-emitting diodes induce autophagy in colon cancer cells by Opsin 3
• 著者・編者: Yoshimoto, Toshiaki; Morine, Yuji; Takasu, Chie; Feng, Rui; Ikemoto, Tetsuya; Yoshikawa, Kozo; Iwahashi, Syuichi; Saito, Yu; Kashihara, Hideya; Akutagawa, Masatake; Emoto, Takahiro; Kinouchi, Yosuke; Shimada, Mitsuo
• 著者標目: Yoshimoto, Toshiaki; Morine, Yuji; Takasu, Chie; Feng, Rui; Ikemoto, Tetsuya; Yoshikawa, Kozo; Iwahashi, Syuichi; Saito, Yu; Kashihara, Hideya; Akutagawa, Masatake; Emoto, Takahiro; Kinouchi, Yosuke; Shimada, Mitsuo
• 出版事項: The Japanese Society of Gastroenterological Surgery|Wiley
• 出版年月日等: 2018-01-11
• 出版年（W3CDTF）: 2018-01-11
• 並列タイトル等: 青色発光ダイオードはオプシン3を介し大腸癌細胞のオートファジーを誘導する
• タイトル（掲載誌）: Annals of Gastroenterological Surgery
• 巻号年月日等（掲載誌）: 2 2
• 掲載巻: 2
• 掲載号: 2
• 掲載ページ: 154-161
• ISSN（掲載誌）: ISSN : 2475-0328
• 授与機関名: 徳島大学
• 授与年月日: 2019-06-27
• 報告番号: 甲第3320号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: autophagy; blue light emitting diodes (LED); colorectal cancer; Opsin 3 (Opn3); photoreceptor
• 対象利用者: 一般
• 一般注記: Light emitting-diodes (LED) have various effects on living organisms and recent studies have shown the efficacy of visible light irradiation from LED for anticancer therapies. However, the mechanism of LED’s effects on cancer cells remains unclear. The aim of the present study was to investigate the effects of LED on colon cancer cell lines and the role of photoreceptor Opsin 3 (Opn3) on LED irradiation in vitro. Human colon cancer cells (HT-29 or HCT-116) were seeded onto laboratory dishes and irradiated with 465-nm LED at 30 mW/cm2 for 30 minutes. Cell Counting Kit-8 was used to measure cell viability, and apoptosis and caspase 3/8 expression were evaluated by AnnexinV/PI and reverse transcription-polymerase chain reaction (RT-PCR), respectively. Autophagy and expression of LC-3 and beclin-1 were also evaluated by autophagy assays, RT-PCR and Western blotting. We further tested Opn3 knockdown by Opn3 siRNA and the Gi/o G-protein inhibitor NF023 in these assays. Viability of HT-29 and HCT-116 cells was lower in 465-nm LED-irradiated cultures than in control cultures. LC-3 and beclin-1 expressions were significantly higher in LED-irradiated cultures, and autophagosomes were detected in irradiated cells. The reductive effect of cancer cell viability following blue LED irradiation was reversed by Opn3 knockdown or NF023 treatment. Furthermore, increased LC-3 and beclin-1 expression that resulted from blue LED irradiation was suppressed by Opn3 knockdown or NF023 treatment. Blue LED irradiation suppressed the growth of colon cancer cells and Opn3 may play an important role as a photoreceptor.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. ( https://creativecommons.org/licenses/by/4.0/ ) © 2018 The Authors. Annals of Gastroenterological Surgery published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Gastroenterological Surgery
• 関連情報（DOI）: 10.1002/ags3.12055
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/113689/20190830170130578905/k3320_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/113689/2019083017013184043/k3320_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/113689/20190830170131455493/k3320_fulltext.pdf fulltext