Blue light-emitting diodes induce autophagy in colon cancer cells by Opsin 3

• Material Type: 博士論文
• Title: Blue light-emitting diodes induce autophagy in colon cancer cells by Opsin 3
• Author/Editor: Yoshimoto, Toshiaki; Morine, Yuji; Takasu, Chie; Feng, Rui; Ikemoto, Tetsuya; Yoshikawa, Kozo; Iwahashi, Syuichi; Saito, Yu; Kashihara, Hideya; Akutagawa, Masatake; Emoto, Takahiro; Kinouchi, Yosuke; Shimada, Mitsuo
• Author Heading: Yoshimoto, Toshiaki; Morine, Yuji; Takasu, Chie; Feng, Rui; Ikemoto, Tetsuya; Yoshikawa, Kozo; Iwahashi, Syuichi; Saito, Yu; Kashihara, Hideya; Akutagawa, Masatake; Emoto, Takahiro; Kinouchi, Yosuke; Shimada, Mitsuo
• Publication, Distribution, etc.: The Japanese Society of Gastroenterological Surgery|Wiley
• Publication Date: 2018-01-11
• Publication Date (W3CDTF): 2018-01-11
• Alternative Title: 青色発光ダイオードはオプシン3を介し大腸癌細胞のオートファジーを誘導する
• Periodical title: Annals of Gastroenterological Surgery
• No. or year of volume/issue: 2 2
• Volume: 2
• Issue: 2
• Pages: 154-161
• ISSN (Periodical Title): ISSN : 2475-0328
• Degree grantor/type: 徳島大学
• Date Granted: 2019-06-27
• Dissertation Number: 甲第3320号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: autophagy; blue light emitting diodes (LED); colorectal cancer; Opsin 3 (Opn3); photoreceptor
• Target Audience: 一般
• Note (General): Light emitting-diodes (LED) have various effects on living organisms and recent studies have shown the efficacy of visible light irradiation from LED for anticancer therapies. However, the mechanism of LED’s effects on cancer cells remains unclear. The aim of the present study was to investigate the effects of LED on colon cancer cell lines and the role of photoreceptor Opsin 3 (Opn3) on LED irradiation in vitro. Human colon cancer cells (HT-29 or HCT-116) were seeded onto laboratory dishes and irradiated with 465-nm LED at 30 mW/cm2 for 30 minutes. Cell Counting Kit-8 was used to measure cell viability, and apoptosis and caspase 3/8 expression were evaluated by AnnexinV/PI and reverse transcription-polymerase chain reaction (RT-PCR), respectively. Autophagy and expression of LC-3 and beclin-1 were also evaluated by autophagy assays, RT-PCR and Western blotting. We further tested Opn3 knockdown by Opn3 siRNA and the Gi/o G-protein inhibitor NF023 in these assays. Viability of HT-29 and HCT-116 cells was lower in 465-nm LED-irradiated cultures than in control cultures. LC-3 and beclin-1 expressions were significantly higher in LED-irradiated cultures, and autophagosomes were detected in irradiated cells. The reductive effect of cancer cell viability following blue LED irradiation was reversed by Opn3 knockdown or NF023 treatment. Furthermore, increased LC-3 and beclin-1 expression that resulted from blue LED irradiation was suppressed by Opn3 knockdown or NF023 treatment. Blue LED irradiation suppressed the growth of colon cancer cells and Opn3 may play an important role as a photoreceptor.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. ( https://creativecommons.org/licenses/by/4.0/ ) © 2018 The Authors. Annals of Gastroenterological Surgery published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Gastroenterological Surgery
• Related Material (DOI): 10.1002/ags3.12055
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/113689/20190830170130578905/k3320_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/113689/2019083017013184043/k3320_review.pdf fulltext

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