The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway

• 資料種別: 博士論文
• タイトル: The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway
• 著者・編者: Takata, Atsushi; Wada, Yuma; Ikemoto, Tetsuya; Morine, Yuji; Imura, Satoru; Iwahashi, Shuichi; Saito, Yu; Shimada, Mitsuo
• 著者標目: Takata, Atsushi; Wada, Yuma; Ikemoto, Tetsuya; Morine, Yuji; Imura, Satoru; Iwahashi, Shuichi; Saito, Yu; Shimada, Mitsuo
• 出版事項: Springer|Japan Surgical Society
• 出版年月日等: 2019-02-07
• 出版年（W3CDTF）: 2019-02-07
• 並列タイトル等: Epigallocatechin 3-gallateのNrf2経路を介したマウス膵島細胞保護作用に関する検討; protective effect of EGCG on mouse islets
• タイトル（掲載誌）: Surgery Today
• 巻号年月日等（掲載誌）: 49 6
• 掲載巻: 49
• 掲載号: 6
• 掲載ページ: 536-545
• ISSN（掲載誌）: ISSN : 0941-1291; ISSN : 1436-2813
• 授与機関名: 徳島大学
• 授与年月日: 2019-12-26
• 報告番号: 甲第3342号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Epigallocatechin 3-gallate (EGCG); Nrf2; islet transplantation; Reactive oxygen species (ROS); Pre-conditioning; Nuclear factor erythroid 2-related factor 2 (Nrf2); Reactive oxygen species (ROS) production
• 対象利用者: 一般
• 一般注記: Purpose: Epigallocatechin 3-gallate (EGCG), a green tea polyphenol, has been shown to have anti-oxidant and anti-inflammatory effects in vitro and in vivo. The aim of this study was to investigate the effects and mechanism of EGCG on isolated pancreatic islets as pre-conditioning for pancreatic islet transplantation. Methods: The pancreatic islets were divided into two groups: an islet culture medium group (control) and an islet culture medium with EGCG (100 μM) group. We investigated the islet viability, Nrf2 expression, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) mRNA. Five hundred islet equivalents after 12 h of culture for the EGCG 100 μM and control group were transplanted under the kidney capsule of streptozotocin (STZ)-induced diabetic ICR mice. Results: The cell viability and insulin secretion ability in the EGCG group were preserved, and the nuclear translocation of Nrf2 was increased in the EGCG group (p<0.01). While the HO-1 mRNA levels were also higher in the EGCG group than in the control group (p<0.05), the ROS production was lower (p<0.01). An in vivo functional assessment showed that the blood glucose level had decreased in the EGCG group after transplantation (p<0.01). Conclusion: EGCG protects the viability and function of islets by suppressing ROS production via the Nrf2 pathway.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1007/s00595-019-1761-0
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/114154/20200116151601706643/k3342_abstract_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/114154/20200403163421958401/k3342_fulltext.pdf fulltext