The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway

• Material Type: 博士論文
• Title: The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway
• Author/Editor: Takata, Atsushi; Wada, Yuma; Ikemoto, Tetsuya; Morine, Yuji; Imura, Satoru; Iwahashi, Shuichi; Saito, Yu; Shimada, Mitsuo
• Author Heading: Takata, Atsushi; Wada, Yuma; Ikemoto, Tetsuya; Morine, Yuji; Imura, Satoru; Iwahashi, Shuichi; Saito, Yu; Shimada, Mitsuo
• Publication, Distribution, etc.: Springer|Japan Surgical Society
• Publication Date: 2019-02-07
• Publication Date (W3CDTF): 2019-02-07
• Alternative Title: Epigallocatechin 3-gallateのNrf2経路を介したマウス膵島細胞保護作用に関する検討; protective effect of EGCG on mouse islets
• Periodical title: Surgery Today
• No. or year of volume/issue: 49 6
• Volume: 49
• Issue: 6
• Pages: 536-545
• ISSN (Periodical Title): ISSN : 0941-1291; ISSN : 1436-2813
• Degree grantor/type: 徳島大学
• Date Granted: 2019-12-26
• Dissertation Number: 甲第3342号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Epigallocatechin 3-gallate (EGCG); Nrf2; islet transplantation; Reactive oxygen species (ROS); Pre-conditioning; Nuclear factor erythroid 2-related factor 2 (Nrf2); Reactive oxygen species (ROS) production
• Target Audience: 一般
• Note (General): Purpose: Epigallocatechin 3-gallate (EGCG), a green tea polyphenol, has been shown to have anti-oxidant and anti-inflammatory effects in vitro and in vivo. The aim of this study was to investigate the effects and mechanism of EGCG on isolated pancreatic islets as pre-conditioning for pancreatic islet transplantation. Methods: The pancreatic islets were divided into two groups: an islet culture medium group (control) and an islet culture medium with EGCG (100 μM) group. We investigated the islet viability, Nrf2 expression, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) mRNA. Five hundred islet equivalents after 12 h of culture for the EGCG 100 μM and control group were transplanted under the kidney capsule of streptozotocin (STZ)-induced diabetic ICR mice. Results: The cell viability and insulin secretion ability in the EGCG group were preserved, and the nuclear translocation of Nrf2 was increased in the EGCG group (p<0.01). While the HO-1 mRNA levels were also higher in the EGCG group than in the control group (p<0.05), the ROS production was lower (p<0.01). An in vivo functional assessment showed that the blood glucose level had decreased in the EGCG group after transplantation (p<0.01). Conclusion: EGCG protects the viability and function of islets by suppressing ROS production via the Nrf2 pathway.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1007/s00595-019-1761-0
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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