Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution

• 資料種別: 博士論文
• タイトル: Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution
• 著者・編者: Sugano, Mikio; Yoshida, Homare; Kurobe, Hirotsugu; Arase, Hiroki; Kinoshita, Hajime; Kitaichi, Takashi; Sugasawa, Noriko; Nakayama, Soichiro; Maeda, Kazuhisa; Irahara, Minoru; Kitagawa, Tetsuya
• 著者標目: Sugano, Mikio; Yoshida, Homare; Kurobe, Hirotsugu; Arase, Hiroki; Kinoshita, Hajime; Kitaichi, Takashi; Sugasawa, Noriko; Nakayama, Soichiro; Maeda, Kazuhisa; Irahara, Minoru; Kitagawa, Tetsuya
• 出版事項: Faculty of Medicine Tokushima University
• 出版年月日等: 2017-02
• 出版年（W3CDTF）: 2017-02
• 並列タイトル等: 免疫欠損肺高血圧モデルマウスに対するヒト臍帯血由来単核球細胞移植の効果; Human cord blood-MNC transplantation improves PH
• タイトル（掲載誌）: The Journal of Medical Investigation
• 巻号年月日等（掲載誌）: 64 1-2
• 掲載巻: 64
• 掲載号: 1-2
• 掲載ページ: 43-49
• ISSN（掲載誌）: ISSN : 1349-6867; ISSN : 1343-1420
• 授与機関名: 徳島大学
• 授与年月日: 2019-12-26
• 報告番号: 乙第2109号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Pulmonary hypertension; cellular therapy; umbilical cord blood; monocrotaline; mononuclear cells
• 対象利用者: 一般
• 一般注記: Objectives : To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. Methods :MCT was administered to BALB/c Slc-nu/nu mice, and PH was induced in mice 4 weeks later. Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice. The medial thickness of pulmonary arterioles, ratio of right ventricular to septum plus left ventricular weight (RV/S+LV), and ratio of acceleration time to ejection time of pulmonary blood flow waveform (AT/ET) were determined 4 weeks after hUCB-MNC transplantation. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung by fluorescent microscopy. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioluminescence images. Results : Medial thickness, RV/S+ LV and AT/ET were significantly improved 4 weeks after hUCB-MNC transplantation compared with those in mice without hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. Conclusions : The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.2152/jmi.64.43
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/114302/20200227092321374188/o2109_abstract_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/114302/20200403133255797752/o2109_fulltext.pdf fulltext