Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution

• Material Type: 博士論文
• Title: Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution
• Author/Editor: Sugano, Mikio; Yoshida, Homare; Kurobe, Hirotsugu; Arase, Hiroki; Kinoshita, Hajime; Kitaichi, Takashi; Sugasawa, Noriko; Nakayama, Soichiro; Maeda, Kazuhisa; Irahara, Minoru; Kitagawa, Tetsuya
• Author Heading: Sugano, Mikio; Yoshida, Homare; Kurobe, Hirotsugu; Arase, Hiroki; Kinoshita, Hajime; Kitaichi, Takashi; Sugasawa, Noriko; Nakayama, Soichiro; Maeda, Kazuhisa; Irahara, Minoru; Kitagawa, Tetsuya
• Publication, Distribution, etc.: Faculty of Medicine Tokushima University
• Publication Date: 2017-02
• Publication Date (W3CDTF): 2017-02
• Alternative Title: 免疫欠損肺高血圧モデルマウスに対するヒト臍帯血由来単核球細胞移植の効果; Human cord blood-MNC transplantation improves PH
• Periodical title: The Journal of Medical Investigation
• No. or year of volume/issue: 64 1-2
• Volume: 64
• Issue: 1-2
• Pages: 43-49
• ISSN (Periodical Title): ISSN : 1349-6867; ISSN : 1343-1420
• Degree grantor/type: 徳島大学
• Date Granted: 2019-12-26
• Dissertation Number: 乙第2109号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Pulmonary hypertension; cellular therapy; umbilical cord blood; monocrotaline; mononuclear cells
• Target Audience: 一般
• Note (General): Objectives : To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. Methods :MCT was administered to BALB/c Slc-nu/nu mice, and PH was induced in mice 4 weeks later. Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice. The medial thickness of pulmonary arterioles, ratio of right ventricular to septum plus left ventricular weight (RV/S+LV), and ratio of acceleration time to ejection time of pulmonary blood flow waveform (AT/ET) were determined 4 weeks after hUCB-MNC transplantation. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung by fluorescent microscopy. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioluminescence images. Results : Medial thickness, RV/S+ LV and AT/ET were significantly improved 4 weeks after hUCB-MNC transplantation compared with those in mice without hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. Conclusions : The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2152/jmi.64.43
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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