Functional analysis of human fibrocytes derived from monocytes reveals their profibrotic phenotype through paracrine effects

• 資料種別: 博士論文
• タイトル: Functional analysis of human fibrocytes derived from monocytes reveals their profibrotic phenotype through paracrine effects
• 著者・編者: Abe, Shuichi; Sato, Seidai; Aono, Yoshinori; Azuma, Momoyo; Kishi, Masami; Koyama, Kazuya; Takahashi, Naoki; Kagawa, Kozo; Kawano, Hiroshi; Nishioka, Yasuhiko
• 著者標目: Abe, Shuichi; Sato, Seidai; Aono, Yoshinori; Azuma, Momoyo; Kishi, Masami; Koyama, Kazuya; Takahashi, Naoki; Kagawa, Kozo; Kawano, Hiroshi; Nishioka, Yasuhiko
• 出版事項: Tokushima University Faculty of Medicine
• 出版年月日等: 2020-02
• 出版年（W3CDTF）: 2020-02
• 並列タイトル等: 単球由来線維細胞のパラクライン効果を介した線維化促進作用の機能解析; Fibrocytes activate fibroblasts by PDGF.
• タイトル（掲載誌）: The Journal of Medical Investigation
• 巻号年月日等（掲載誌）: 67 1-2
• 掲載巻: 67
• 掲載号: 1-2
• 掲載ページ: 102-112
• ISSN（掲載誌）: ISSN : 1349-6867; ISSN : 1343-1420
• 授与機関名: 徳島大学
• 授与年月日: 2020-03-05
• 報告番号: 甲第3363号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: fibrocyte; fibroblast; idiopathic pulmonary fibrosis; PDGF
• 対象利用者: 一般
• 一般注記: Fibrocytes, which are bone marrow-derived collagen-producing cells, were reported to play a role in the pathogenesis of pulmonary fibrosis. However, their function in pulmonary fibrosis is unclear. We analyzed their function compared with that of monocytes and localization in fibrotic tissues in patients with idiopathic pulmonary fibrosis (IPF). We compared the gene expression profile of monocyte-derived fibrocytes with that of monocytes by microarray analysis. Proliferation and differentiation into myofibroblasts were examined by 3H-thymidine incorporation assay and Western blotting. We measured the level of growth factors in the culture supernatant of fibrocytes by ELISA. The localization of fibrocytes in lung tissues of patients with IPF was determined by immunofluorescence staining. Compared with monocytes, fibrocytes had higher expression of extracellular matrix- and growth factor-encoding genes, including PDGF-B, FGF-2 and VEGF-B. Although fibrocytes did not proliferate in response to PDGF, co-culture of fibrocytes stimulated the growth of lung fibroblasts through the production of PDGF-BB. In the lung of IPF patients, CD45+Collagen-I+FSP-1+ cells, which have a similar phenotype to fibrocytes, were detected and co-stained with anti-PDGF antibody. This study suggested that fibrocytes function in pulmonary fibrosis partly by producing PDGF in the lungs of IPF patients.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.2152/jmi.67.102
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/114734/20200514115355785830/k3363_abstract_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/114734/20200610104313154828/k3363_fulltext.pdf fulltext