Functional analysis of human fibrocytes derived from monocytes reveals their profibrotic phenotype through paracrine effects

• Material Type: 博士論文
• Title: Functional analysis of human fibrocytes derived from monocytes reveals their profibrotic phenotype through paracrine effects
• Author/Editor: Abe, Shuichi; Sato, Seidai; Aono, Yoshinori; Azuma, Momoyo; Kishi, Masami; Koyama, Kazuya; Takahashi, Naoki; Kagawa, Kozo; Kawano, Hiroshi; Nishioka, Yasuhiko
• Author Heading: Abe, Shuichi; Sato, Seidai; Aono, Yoshinori; Azuma, Momoyo; Kishi, Masami; Koyama, Kazuya; Takahashi, Naoki; Kagawa, Kozo; Kawano, Hiroshi; Nishioka, Yasuhiko
• Publication, Distribution, etc.: Tokushima University Faculty of Medicine
• Publication Date: 2020-02
• Publication Date (W3CDTF): 2020-02
• Alternative Title: 単球由来線維細胞のパラクライン効果を介した線維化促進作用の機能解析; Fibrocytes activate fibroblasts by PDGF.
• Periodical title: The Journal of Medical Investigation
• No. or year of volume/issue: 67 1-2
• Volume: 67
• Issue: 1-2
• Pages: 102-112
• ISSN (Periodical Title): ISSN : 1349-6867; ISSN : 1343-1420
• Degree grantor/type: 徳島大学
• Date Granted: 2020-03-05
• Dissertation Number: 甲第3363号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: fibrocyte; fibroblast; idiopathic pulmonary fibrosis; PDGF
• Target Audience: 一般
• Note (General): Fibrocytes, which are bone marrow-derived collagen-producing cells, were reported to play a role in the pathogenesis of pulmonary fibrosis. However, their function in pulmonary fibrosis is unclear. We analyzed their function compared with that of monocytes and localization in fibrotic tissues in patients with idiopathic pulmonary fibrosis (IPF). We compared the gene expression profile of monocyte-derived fibrocytes with that of monocytes by microarray analysis. Proliferation and differentiation into myofibroblasts were examined by 3H-thymidine incorporation assay and Western blotting. We measured the level of growth factors in the culture supernatant of fibrocytes by ELISA. The localization of fibrocytes in lung tissues of patients with IPF was determined by immunofluorescence staining. Compared with monocytes, fibrocytes had higher expression of extracellular matrix- and growth factor-encoding genes, including PDGF-B, FGF-2 and VEGF-B. Although fibrocytes did not proliferate in response to PDGF, co-culture of fibrocytes stimulated the growth of lung fibroblasts through the production of PDGF-BB. In the lung of IPF patients, CD45+Collagen-I+FSP-1+ cells, which have a similar phenotype to fibrocytes, were detected and co-stained with anti-PDGF antibody. This study suggested that fibrocytes function in pulmonary fibrosis partly by producing PDGF in the lungs of IPF patients.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2152/jmi.67.102
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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