Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticu

• 資料種別: 博士論文
• タイトル: Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticu
• 著者・編者: Maetani-Yasui, Miki; Mawatari, Kazuaki; Honjo, Airi; Bui, Thi Kim Ngan; Shimohata, Takaaki; Uebanso, Takashi; Aihara, Mutsumi; Emoto, Takahiro; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• 著者標目: Maetani-Yasui, Miki; Mawatari, Kazuaki; Honjo, Airi; Bui, Thi Kim Ngan; Shimohata, Takaaki; Uebanso, Takashi; Aihara, Mutsumi; Emoto, Takahiro; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• 出版事項: MDPI
• 出版年月日等: 2020-08-11
• 出版年（W3CDTF）: 2020-08-11
• 並列タイトル等: 腸炎ビブリオにおけるトランスポゾン変異株を用いた長波長紫外線（UVA）感受性に関連する遺伝子の同定; Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticus
• タイトル（掲載誌）: Applied Sciences
• 巻号年月日等（掲載誌）: 10 16
• 掲載巻: 10
• 掲載号: 16
• 掲載ページ: 5549-
• ISSN（掲載誌）: ISSN : 2076-3417
• 授与機関名: 徳島大学
• 授与年月日: 2020-09-24
• 報告番号: 甲第3462号
• 学位: 博士（栄養学）
• 本文の言語コード: eng
• 件名標目: Vibrio parahaemolyticus; ultraviolet; light-emitting diodes; UV-LED; Oxidative stress; light-emitting diode; ultraviolet A
• 対象利用者: 一般
• 一般注記: Ultraviolet (UV) irradiation is used to disinfect water and food and can be classified as UVA (detected at wavelengths 320–400 nm), UVB (280–320 nm), and UVC (<280 nm). We developed a method for UVA sterilization of equipment with a UVA-light-emitting diode (LED); however, a high rate of fluence was needed to promote pathogen inactivation. The aim of this study was to identify genes associated with UVA sensitivity with the goal of improving UVA-LED-mediated bactericidal activity. We constructed a transposon-mutant library of Vibrio parahaemolyticus and selected six mutants with high sensitivity to UVA irradiation. Genes associated with this phenotype include F-type H+-transporting ATPases (atp), as well as those involved in general secretion (gsp), and ubiquinone and terpenoid-quinone biosynthesis (ubi). Gene complementation resulted in decreased sensitivity to UVA-LED. The atp mutants had lower intracellular adenosine triphosphate (ATP) concentrations than the wild-type treatment, with 20 mM L-serine resulting in elevated ATP concentrations and decreased sensitivity to UVA-LED. The gsp mutants exhibited high levels of extracellular protein transport and the ubi mutants exhibited significantly different intracellular concentrations of ubiquinone-8. Taken together, our results suggest that the protein products of the atp, gsp, and ubi genes may regulate sensitivity to UVA irradiation.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• 関連情報（DOI）: 10.3390/app10165549
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/115319/20201001144325420902/k3462_abstract_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/115319/20201001144325989749/k3462_fulltext.pdf fulltext