Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticu

• Material Type: 博士論文
• Title: Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticu
• Author/Editor: Maetani-Yasui, Miki; Mawatari, Kazuaki; Honjo, Airi; Bui, Thi Kim Ngan; Shimohata, Takaaki; Uebanso, Takashi; Aihara, Mutsumi; Emoto, Takahiro; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• Author Heading: Maetani-Yasui, Miki; Mawatari, Kazuaki; Honjo, Airi; Bui, Thi Kim Ngan; Shimohata, Takaaki; Uebanso, Takashi; Aihara, Mutsumi; Emoto, Takahiro; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• Publication, Distribution, etc.: MDPI
• Publication Date: 2020-08-11
• Publication Date (W3CDTF): 2020-08-11
• Alternative Title: 腸炎ビブリオにおけるトランスポゾン変異株を用いた長波長紫外線（UVA）感受性に関連する遺伝子の同定; Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticus
• Periodical title: Applied Sciences
• No. or year of volume/issue: 10 16
• Volume: 10
• Issue: 16
• Pages: 5549-
• ISSN (Periodical Title): ISSN : 2076-3417
• Degree grantor/type: 徳島大学
• Date Granted: 2020-09-24
• Dissertation Number: 甲第3462号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: Vibrio parahaemolyticus; ultraviolet; light-emitting diodes; UV-LED; Oxidative stress; light-emitting diode; ultraviolet A
• Target Audience: 一般
• Note (General): Ultraviolet (UV) irradiation is used to disinfect water and food and can be classified as UVA (detected at wavelengths 320–400 nm), UVB (280–320 nm), and UVC (<280 nm). We developed a method for UVA sterilization of equipment with a UVA-light-emitting diode (LED); however, a high rate of fluence was needed to promote pathogen inactivation. The aim of this study was to identify genes associated with UVA sensitivity with the goal of improving UVA-LED-mediated bactericidal activity. We constructed a transposon-mutant library of Vibrio parahaemolyticus and selected six mutants with high sensitivity to UVA irradiation. Genes associated with this phenotype include F-type H+-transporting ATPases (atp), as well as those involved in general secretion (gsp), and ubiquinone and terpenoid-quinone biosynthesis (ubi). Gene complementation resulted in decreased sensitivity to UVA-LED. The atp mutants had lower intracellular adenosine triphosphate (ATP) concentrations than the wild-type treatment, with 20 mM L-serine resulting in elevated ATP concentrations and decreased sensitivity to UVA-LED. The gsp mutants exhibited high levels of extracellular protein transport and the ubi mutants exhibited significantly different intracellular concentrations of ubiquinone-8. Taken together, our results suggest that the protein products of the atp, gsp, and ubi genes may regulate sensitivity to UVA irradiation.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Rights (production): © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• Related Material (DOI): 10.3390/app10165549
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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