Isorhamnetin, a 3'-methoxylated flavonol, enhances the lysosomal proteolysis in J774.1 murine macrophages in a TFEB-independent manner.

• 資料種別: 博士論文
• タイトル: Isorhamnetin, a 3'-methoxylated flavonol, enhances the lysosomal proteolysis in J774.1 murine macrophages in a TFEB-independent manner.
• 著者・編者: Sakai, Maiko; Ohnishi, Kohta; Masuda, Masashi; Ohminami, Hirokazu; Yamanaka-Okumura, Hisami; Hara, Taichi; Taketani, Yutaka
• 著者標目: Sakai, Maiko; Ohnishi, Kohta; Masuda, Masashi; Ohminami, Hirokazu; Yamanaka-Okumura, Hisami; Hara, Taichi; Taketani, Yutaka
• 出版事項: Japan Society for Bioscience, Biotechnology, and Agrochemistry|Taylor & Francis
• 出版年月日等: 2020-06-02
• 出版年（W3CDTF）: 2020-06-02
• 並列タイトル等: イソラムネチンはJ774.1マウスマクロファージ様細胞株においてTFEB非依存的にリソソームのタンパク質分解を促進する; Isorhamnetin activates lysosomes in J774.1 macrophages.
• タイトル（掲載誌）: Bioscience, Biotechnology, and Biochemistry
• 巻号年月日等（掲載誌）: 84 6
• 掲載巻: 84
• 掲載号: 6
• 掲載ページ: 1221-1231
• ISSN（掲載誌）: ISSN : 0916-8451; ISSN : 1347-6947
• 授与機関名: 徳島大学
• 授与年月日: 2021-03-23
• 報告番号: 甲第3509号
• 学位: 博士（栄養学）
• 本文の言語コード: eng
• 件名標目: Isorhamnetin; TFEB; lysosome; macrophage; polyphenol
• 対象利用者: 一般
• 一般注記: Lysosome is the principal organelle for the ultimate degradation of cellular macromolecules, which are delivered through endocytosis, phagocytosis and autophagy. The lysosomal functions have been found to be impaired by fatty foods and aging, and more importantly, the lysosomal dysfunction in macrophages has been reported as a risk of atherosclerosis development. In this study, we searched for dietary polyphenols which possess the activity for enhancing the lysosomal degradation in J774.1, a murine macrophage-like cell line. Screening test utilizing DQ-BSA digestion identified isorhamnetin (3’-O-methylquercetin) as an active compound. Interestingly, structural comparison to inactive flavonols revealed that the chemical structure of the B-ring moiety in isorhamnetin is the primary determinant of its lysosome-enhancing activity. Unexpectedly isorhamnetin failed to inhibit mTORC1-TFEB signaling, a master regulator of lysosomal biogenesis and function. Our data suggested that the other molecular mechanism might be critical for the regulation of lysosomes in macrophages.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1080/09168451.2020.1727309
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/116062/20210617102722543427/k3509_abstract_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/116062/2021061710272332764/k3509_fulltext.pdf fulltext