Isorhamnetin, a 3'-methoxylated flavonol, enhances the lysosomal proteolysis in J774.1 murine macrophages in a TFEB-independent manner.

• Material Type: 博士論文
• Title: Isorhamnetin, a 3'-methoxylated flavonol, enhances the lysosomal proteolysis in J774.1 murine macrophages in a TFEB-independent manner.
• Author/Editor: Sakai, Maiko; Ohnishi, Kohta; Masuda, Masashi; Ohminami, Hirokazu; Yamanaka-Okumura, Hisami; Hara, Taichi; Taketani, Yutaka
• Author Heading: Sakai, Maiko; Ohnishi, Kohta; Masuda, Masashi; Ohminami, Hirokazu; Yamanaka-Okumura, Hisami; Hara, Taichi; Taketani, Yutaka
• Publication, Distribution, etc.: Japan Society for Bioscience, Biotechnology, and Agrochemistry|Taylor & Francis
• Publication Date: 2020-06-02
• Publication Date (W3CDTF): 2020-06-02
• Alternative Title: イソラムネチンはJ774.1マウスマクロファージ様細胞株においてTFEB非依存的にリソソームのタンパク質分解を促進する; Isorhamnetin activates lysosomes in J774.1 macrophages.
• Periodical title: Bioscience, Biotechnology, and Biochemistry
• No. or year of volume/issue: 84 6
• Volume: 84
• Issue: 6
• Pages: 1221-1231
• ISSN (Periodical Title): ISSN : 0916-8451; ISSN : 1347-6947
• Degree grantor/type: 徳島大学
• Date Granted: 2021-03-23
• Dissertation Number: 甲第3509号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: Isorhamnetin; TFEB; lysosome; macrophage; polyphenol
• Target Audience: 一般
• Note (General): Lysosome is the principal organelle for the ultimate degradation of cellular macromolecules, which are delivered through endocytosis, phagocytosis and autophagy. The lysosomal functions have been found to be impaired by fatty foods and aging, and more importantly, the lysosomal dysfunction in macrophages has been reported as a risk of atherosclerosis development. In this study, we searched for dietary polyphenols which possess the activity for enhancing the lysosomal degradation in J774.1, a murine macrophage-like cell line. Screening test utilizing DQ-BSA digestion identified isorhamnetin (3’-O-methylquercetin) as an active compound. Interestingly, structural comparison to inactive flavonols revealed that the chemical structure of the B-ring moiety in isorhamnetin is the primary determinant of its lysosome-enhancing activity. Unexpectedly isorhamnetin failed to inhibit mTORC1-TFEB signaling, a master regulator of lysosomal biogenesis and function. Our data suggested that the other molecular mechanism might be critical for the regulation of lysosomes in macrophages.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1080/09168451.2020.1727309
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/116062/20210617102722543427/k3509_abstract_review.pdf fulltext

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