UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength

• 資料種別: 博士論文
• タイトル: UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength
• 著者・編者: Bui, Thi Kim Ngan; Mawatari, Kazuaki; Emoto, Takahiro; Fukushima, Shiho; Shimohata, Takaaki; Uebanso, Takashi; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• 著者標目: Bui, Thi Kim Ngan; Mawatari, Kazuaki; Emoto, Takahiro; Fukushima, Shiho; Shimohata, Takaaki; Uebanso, Takashi; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• 出版事項: Elsevier
• 出版年月日等: 2022-02-14
• 出版年（W3CDTF）: 2022-02-14
• 並列タイトル等: 紫外線発光ダイオードの照射は、そのピーク波長によって異なるウイルス分子を標的にすることで単純ヘルペスウイルスの感染性を減弱する
• タイトル（掲載誌）: Journal of Photochemistry and Photobiology B: Biology
• 巻号年月日等（掲載誌）: 228
• 掲載巻: 228
• 掲載ページ: 112410-
• ISSN（掲載誌）: ISSN : 1011-1344
• 授与機関名: 徳島大学
• 授与年月日: 2022-03-23
• 報告番号: 甲第3602号
• 学位: 博士（栄養学）
• 本文の言語コード: eng
• 件名標目: UV-LED; herpes simplex virus type 1; DNA photoproducs; viral lipids; Light-emitting diode; Ultraviolet
• 対象利用者: 一般
• 一般注記: Herpes simplex virus type 1 (HSV-1) is an enveloped virus that mainly infects humans. Given its high global prevalence, disinfection is critical for reducing the risk of infection. Ultraviolet-light-emitting diodes (UV-LEDs) are eco-friendly irradiating modules with different peak wavelengths, but the molecules degraded by UV-LED irradiation have not been clarified. To identify the target viral molecules of UV-LEDs, we exposed HSV-1 suspensions to UV-LED irradiation at wavelengths of 260-, 280-, 310-, and 365-nm and measured viral DNA, protein, and lipid damage and infectivity in host cells. All UV-LEDs substantially reduced by inhibiting host cell transcription, but 260- and 280-nm UV-LEDs had significantly stronger virucidal efficiency than 310- and 365-nm UV-LEDs. Meanwhile, 260- and 280-nm UV-LEDs induced the formation of viral DNA photoproducts and the degradation of viral proteins and some phosphoglycerolipid species. Unlike 260- and 280-nm UV-LEDs, 310- and 365-nm UV-LEDs decreased the viral protein levels, but they did not drastically change the levels of viral DNA photoproducts and lipophilic metabolites. These results suggest that UV-LEDs reduce the infectivity of HSV-1 by targeting different viral molecules based on the peak wavelength. These findings could facilitate the optimization of UV-LED irradiation for viral inactivation.
• 記録形式（IMT）: application/pdf
• 一次資料へのリンクURL: comment：fulltext; http://repo.lib.tokushima-u.ac.jp/files/public/11/117004/2022051015043933686/k3602_abstract_review.pdf; http://repo.lib.tokushima-u.ac.jp/files/public/11/117004/20220510150439357754/k3602_fulltext.pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1016/j.jphotobiol.2022.112410
• 掲載誌（NCID）: AA10686173; https://ci.nii.ac.jp/ncid/AA10686173; AA11533983; https://ci.nii.ac.jp/ncid/AA11533983
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ