UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength

• Material Type: 博士論文
• Title: UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength
• Author/Editor: Bui, Thi Kim Ngan; Mawatari, Kazuaki; Emoto, Takahiro; Fukushima, Shiho; Shimohata, Takaaki; Uebanso, Takashi; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• Author Heading: Bui, Thi Kim Ngan; Mawatari, Kazuaki; Emoto, Takahiro; Fukushima, Shiho; Shimohata, Takaaki; Uebanso, Takashi; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2022-02-14
• Publication Date (W3CDTF): 2022-02-14
• Alternative Title: 紫外線発光ダイオードの照射は、そのピーク波長によって異なるウイルス分子を標的にすることで単純ヘルペスウイルスの感染性を減弱する
• Periodical title: Journal of Photochemistry and Photobiology B: Biology
• No. or year of volume/issue: 228
• Volume: 228
• Pages: 112410-
• ISSN (Periodical Title): ISSN : 1011-1344
• Degree grantor/type: 徳島大学
• Date Granted: 2022-03-23
• Dissertation Number: 甲第3602号
• Degree Type: 博士（栄養学）
• Text Language Code: eng
• Subject Heading: UV-LED; herpes simplex virus type 1; DNA photoproducs; viral lipids; Light-emitting diode; Ultraviolet
• Target Audience: 一般
• Note (General): Herpes simplex virus type 1 (HSV-1) is an enveloped virus that mainly infects humans. Given its high global prevalence, disinfection is critical for reducing the risk of infection. Ultraviolet-light-emitting diodes (UV-LEDs) are eco-friendly irradiating modules with different peak wavelengths, but the molecules degraded by UV-LED irradiation have not been clarified. To identify the target viral molecules of UV-LEDs, we exposed HSV-1 suspensions to UV-LED irradiation at wavelengths of 260-, 280-, 310-, and 365-nm and measured viral DNA, protein, and lipid damage and infectivity in host cells. All UV-LEDs substantially reduced by inhibiting host cell transcription, but 260- and 280-nm UV-LEDs had significantly stronger virucidal efficiency than 310- and 365-nm UV-LEDs. Meanwhile, 260- and 280-nm UV-LEDs induced the formation of viral DNA photoproducts and the degradation of viral proteins and some phosphoglycerolipid species. Unlike 260- and 280-nm UV-LEDs, 310- and 365-nm UV-LEDs decreased the viral protein levels, but they did not drastically change the levels of viral DNA photoproducts and lipophilic metabolites. These results suggest that UV-LEDs reduce the infectivity of HSV-1 by targeting different viral molecules based on the peak wavelength. These findings could facilitate the optimization of UV-LED irradiation for viral inactivation.
• Format (IMT): application/pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.1016/j.jphotobiol.2022.112410
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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http://repo.lib.tokushima-u.ac.jp/files/public/11/117004/2022051015043933686/k3602_abstract_review.pdf fulltext

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