Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways

• 資料種別: 博士論文
• タイトル: Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways
• 著者・編者: Uemura, Yuta; Hiroshima, Yuka; Tada, Ayano; Murakami, Keiji; Yoshida, Kaya; Inagaki, Yuji; Kuwahara, Tomomi; Murakami, Akikazu; Fujii, Hideki; Yumoto, Hiromichi
• 著者標目: Uemura, Yuta; Hiroshima, Yuka; Tada, Ayano; Murakami, Keiji; Yoshida, Kaya; Inagaki, Yuji; Kuwahara, Tomomi; Murakami, Akikazu; Fujii, Hideki; Yumoto, Hiromichi
• 出版事項: MDPI
• 出版年月日等: 2022-10-20
• 出版年（W3CDTF）: 2022-10-20
• 並列タイトル等: Porphyromonas gingivalisの外膜小胞は歯肉上皮細胞を刺激しMAPKとSTING経路を介して炎症性サイトカインを産生させる
• タイトル（掲載誌）: Biomedicines
• 巻号年月日等（掲載誌）: 10 10
• 掲載巻: 10
• 掲載号: 10
• 掲載ページ: 2643-
• ISSN（掲載誌）: ISSN : 2227-9059
• 授与機関名: 徳島大学
• 授与年月日: 2023-03-23
• 報告番号: 甲第3712号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: Porphyromonas gingivalis; outer membrane vesicles; stimulator of interferon genes; inflammation; periodontitis
• 対象利用者: 一般
• 一般注記: Porphyromonas gingivalis (Pg) is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (OMVs) that contain lipopolysaccharide (LPS), gingipains, and pathogen-derived DNA and RNA. Pg-OMVs are involved in the pathogenesis of periodontitis. Pg-OMV-activated pathways that induce the production of the pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in the human gingival epithelial cell line, OBA-9, were investigated. The role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB in levels of Pg-OMV-induced pro-inflammatory cytokines was investigated using Western blot analysis and specific pathway inhibitors. Pg-OMVs induced IL-6 and IL-8 production via the extracellular signal-regulated kinase (Erk) 1/2, c-Jun N-terminal kinase (JNK), p38 MAPK, and NF-κB signaling pathways in OBA-9 cells. In addition, the stimulator of interferon genes (STING), an essential innate immune signaling molecule, was triggered by a cytosolic pathogen DNA. Pg-OMV-induced IL-6 and IL-8 mRNA expression and production were significantly suppressed by STING-specific small interfering RNA. Taken together, these results demonstrated that Pg-OMV-activated Erk1/2, JNK, p38 MAPK, STING, and NF-κB signaling pathways resulting in increased IL-6 and IL-8 expression in human gingival epithelial cells. These results suggest that Pg-OMVs may play important roles in periodontitis exacerbation by stimulating various pathways.
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• 関連情報（DOI）: 10.3390/biomedicines10102643
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/117616/20230609152640913604/k3712_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/117616/2023060915264157026/k3712_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/117616/20230609152641158471/k3712_fulltext.pdf fulltext