Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways

• Material Type: 博士論文
• Title: Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways
• Author/Editor: Uemura, Yuta; Hiroshima, Yuka; Tada, Ayano; Murakami, Keiji; Yoshida, Kaya; Inagaki, Yuji; Kuwahara, Tomomi; Murakami, Akikazu; Fujii, Hideki; Yumoto, Hiromichi
• Author Heading: Uemura, Yuta; Hiroshima, Yuka; Tada, Ayano; Murakami, Keiji; Yoshida, Kaya; Inagaki, Yuji; Kuwahara, Tomomi; Murakami, Akikazu; Fujii, Hideki; Yumoto, Hiromichi
• Publication, Distribution, etc.: MDPI
• Publication Date: 2022-10-20
• Publication Date (W3CDTF): 2022-10-20
• Alternative Title: Porphyromonas gingivalisの外膜小胞は歯肉上皮細胞を刺激しMAPKとSTING経路を介して炎症性サイトカインを産生させる
• Periodical title: Biomedicines
• No. or year of volume/issue: 10 10
• Volume: 10
• Issue: 10
• Pages: 2643-
• ISSN (Periodical Title): ISSN : 2227-9059
• Degree grantor/type: 徳島大学
• Date Granted: 2023-03-23
• Dissertation Number: 甲第3712号
• Degree Type: 博士（歯学）
• Text Language Code: eng
• Subject Heading: Porphyromonas gingivalis; outer membrane vesicles; stimulator of interferon genes; inflammation; periodontitis
• Target Audience: 一般
• Note (General): Porphyromonas gingivalis (Pg) is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (OMVs) that contain lipopolysaccharide (LPS), gingipains, and pathogen-derived DNA and RNA. Pg-OMVs are involved in the pathogenesis of periodontitis. Pg-OMV-activated pathways that induce the production of the pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in the human gingival epithelial cell line, OBA-9, were investigated. The role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB in levels of Pg-OMV-induced pro-inflammatory cytokines was investigated using Western blot analysis and specific pathway inhibitors. Pg-OMVs induced IL-6 and IL-8 production via the extracellular signal-regulated kinase (Erk) 1/2, c-Jun N-terminal kinase (JNK), p38 MAPK, and NF-κB signaling pathways in OBA-9 cells. In addition, the stimulator of interferon genes (STING), an essential innate immune signaling molecule, was triggered by a cytosolic pathogen DNA. Pg-OMV-induced IL-6 and IL-8 mRNA expression and production were significantly suppressed by STING-specific small interfering RNA. Taken together, these results demonstrated that Pg-OMV-activated Erk1/2, JNK, p38 MAPK, STING, and NF-κB signaling pathways resulting in increased IL-6 and IL-8 expression in human gingival epithelial cells. These results suggest that Pg-OMVs may play important roles in periodontitis exacerbation by stimulating various pathways.
• Access Restrictions: インターネット公開
• Rights (production): This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
• Related Material (DOI): 10.3390/biomedicines10102643
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ

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