Effects of activation with a Ca ionophore and roscovitine on the development of human oocytes that failed to fertilize after ICSI

• 資料種別: 博士論文
• タイトル: Effects of activation with a Ca ionophore and roscovitine on the development of human oocytes that failed to fertilize after ICSI
• 著者・編者: Suto, Ayako; Yano, Yuya; Yamamoto, Yuri; Noguchi, Hiroki; Takeda, Asuka; Yamamoto, Shota; Kagawa, Tomohiro; Yoshida, Kanako; Hinokio, Kenji; Kuwahara, Akira; Yasui, Toshiyuki; Iwasa, Takeshi
• 著者標目: Suto, Ayako; Yano, Yuya; Yamamoto, Yuri; Noguchi, Hiroki; Takeda, Asuka; Yamamoto, Shota; Kagawa, Tomohiro; Yoshida, Kanako; Hinokio, Kenji; Kuwahara, Akira; Yasui, Toshiyuki; Iwasa, Takeshi
• 出版事項: Tokushima University Faculty of Medicine
• 出版年月日等: 2023-08
• 出版年（W3CDTF）: 2023-08
• 並列タイトル等: 顕微授精後のヒト未受精卵に対するカルシウムイオノファとロスコビチンを併用した卵活性化の有用性; Activation with a Ca ionophore and roscovitine is effective in terms of fertilization, especially in the TESE-ICSI treated cases.
• タイトル（掲載誌）: The Journal of Medical Investigation
• 巻号年月日等（掲載誌）: 70 3-4
• 掲載巻: 70
• 掲載号: 3-4
• 掲載ページ: 321-324
• ISSN（掲載誌）: ISSN : 1349-6867; ISSN : 1343-1420
• 授与機関名: 徳島大学
• 授与年月日: 2023-04-27
• 報告番号: 甲第3739号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Ca ionophore; roscovitine; ICSI; oocyte activation; TESE
• 対象利用者: 一般
• 一般注記: Objective : The effects of oocyte activation with a Ca ionophore and roscovitine (Ca+R), a selective inhibitor of M-phase promoting factor, on unfertilized oocytes after intracytoplasmic sperm injection (ICSI) or testicular sperm extraction (TESE)-ICSI were evaluated. Method : Oocytes without pronuclei at 18 hours after ICSI were judged to be unfertilized and were exposed to the Ca ionophore A23187 (5 μM) with or without roscovitine (50 μM). The activation rate was measured 3, 7, and 18 hours later. Oocytes with two polar bodies and two pronuclei with a sperm tail were judged to have been activated. Results : At 18 hours, the activation rates in the control, Ca ionophore, and Ca+R groups were 3.5% (4 / 112), 26.9% (7 / 26), and 32.1% (17 / 53), respectively. The activation rate of the Ca+R group was significantly higher than that of the control and similar to that of the Ca ionophore group. Among the oocytes that remained unfertilized after TESE-ICSI, the activation rates of the Ca ionophore and Ca+R groups were 22.2% (2 / 9) and 43.8% (7 / 16), respectively. Conclusions : Sequential treatment with an Ca ionophore and roscovitine activates oocytes that remain unfertilized after ICSI. In TESE-ICSI, the activation rate tended to be increased by the co-administration of roscovitine with a Ca ionophore.
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.2152/jmi.70.321
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/118372/20230630145411397775/k3739_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/118372/20230630145411493805/k3739_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/118372/20231201105805881337/k3739_fulltext.pdf fulltext