Effects of activation with a Ca ionophore and roscovitine on the development of human oocytes that failed to fertilize after ICSI

• Material Type: 博士論文
• Title: Effects of activation with a Ca ionophore and roscovitine on the development of human oocytes that failed to fertilize after ICSI
• Author/Editor: Suto, Ayako; Yano, Yuya; Yamamoto, Yuri; Noguchi, Hiroki; Takeda, Asuka; Yamamoto, Shota; Kagawa, Tomohiro; Yoshida, Kanako; Hinokio, Kenji; Kuwahara, Akira; Yasui, Toshiyuki; Iwasa, Takeshi
• Author Heading: Suto, Ayako; Yano, Yuya; Yamamoto, Yuri; Noguchi, Hiroki; Takeda, Asuka; Yamamoto, Shota; Kagawa, Tomohiro; Yoshida, Kanako; Hinokio, Kenji; Kuwahara, Akira; Yasui, Toshiyuki; Iwasa, Takeshi
• Publication, Distribution, etc.: Tokushima University Faculty of Medicine
• Publication Date: 2023-08
• Publication Date (W3CDTF): 2023-08
• Alternative Title: 顕微授精後のヒト未受精卵に対するカルシウムイオノファとロスコビチンを併用した卵活性化の有用性; Activation with a Ca ionophore and roscovitine is effective in terms of fertilization, especially in the TESE-ICSI treated cases.
• Periodical title: The Journal of Medical Investigation
• No. or year of volume/issue: 70 3-4
• Volume: 70
• Issue: 3-4
• Pages: 321-324
• ISSN (Periodical Title): ISSN : 1349-6867; ISSN : 1343-1420
• Degree grantor/type: 徳島大学
• Date Granted: 2023-04-27
• Dissertation Number: 甲第3739号
• Degree Type: 博士（医学）
• Text Language Code: eng
• Subject Heading: Ca ionophore; roscovitine; ICSI; oocyte activation; TESE
• Target Audience: 一般
• Note (General): Objective : The effects of oocyte activation with a Ca ionophore and roscovitine (Ca+R), a selective inhibitor of M-phase promoting factor, on unfertilized oocytes after intracytoplasmic sperm injection (ICSI) or testicular sperm extraction (TESE)-ICSI were evaluated. Method : Oocytes without pronuclei at 18 hours after ICSI were judged to be unfertilized and were exposed to the Ca ionophore A23187 (5 μM) with or without roscovitine (50 μM). The activation rate was measured 3, 7, and 18 hours later. Oocytes with two polar bodies and two pronuclei with a sperm tail were judged to have been activated. Results : At 18 hours, the activation rates in the control, Ca ionophore, and Ca+R groups were 3.5% (4 / 112), 26.9% (7 / 26), and 32.1% (17 / 53), respectively. The activation rate of the Ca+R group was significantly higher than that of the control and similar to that of the Ca ionophore group. Among the oocytes that remained unfertilized after TESE-ICSI, the activation rates of the Ca ionophore and Ca+R groups were 22.2% (2 / 9) and 43.8% (7 / 16), respectively. Conclusions : Sequential treatment with an Ca ionophore and roscovitine activates oocytes that remain unfertilized after ICSI. In TESE-ICSI, the activation rate tended to be increased by the co-administration of roscovitine with a Ca ionophore.
• Source: fulltext; http://repo.lib.tokushima-u.ac.jp/files/public/11/118372/20230630145411397775/k3739_abstract.pdf; http://repo.lib.tokushima-u.ac.jp/files/public/11/118372/20230630145411493805/k3739_review.pdf; http://repo.lib.tokushima-u.ac.jp/files/public/11/118372/20231201105805881337/k3739_fulltext.pdf
• Access Restrictions: インターネット公開
• Related Material (DOI): 10.2152/jmi.70.321
• Periodical Title (NCID): AA11166929; https://ci.nii.ac.jp/ncid/AA11166929
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 徳島大学 : 徳島大学機関リポジトリ