一般注記We described a method to detect α2-3 linked and α2-6 linked sialic acids on the cell surface with using flow cytometry. Cells were fixed with 4% paraformaldehyde, and then α2-3 and α2-6 sialic acids were stained with biotinylated MAACKIA AMURENSIS LECTIN II (MALII) and biotinylated ELDERBERRY BARK LECTIN (SNA), respectively. Sialic acids on the cell surface were cleaved by sialidase in acetate buffer at pH 5.5 to confirm the specificity of staining. Streptavidin conjugated with Alexa flour 488 was used to detect biotinylated lectins. Thus, the α2-3 linked and α2-6 linked sialic acids on the cell surface were semi-quantitatively detected by flow cytometry.
Methods in Molecular Biology book series (MIMB,volume 2556)Springer Protocols
source:https://doi.org/10.1007/978-1-0716-2635-1_3
著作権情報© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）
提供元機関・データベース滋賀医科大学 : 滋賀医科大学機関リポジトリびわ庫