Detection of Sialic Acids on the Cell Surface Using Flow Cytometry

• Material Type: 図書
• Title: Detection of Sialic Acids on the Cell Surface Using Flow Cytometry
• Author/Editor: 石垣, 宏仁; 伊藤, 靖; Hirohito, ISHIGAKI; Yasushi, ITOH
• Author Heading: 石垣, 宏仁; 伊藤, 靖; Hirohito, ISHIGAKI; Yasushi, ITOH
• Publication, Distribution, etc.: Humana Press Inc.
• Publication Date: 2022-09-30
• Publication Date (W3CDTF): 2022-09-30
• Periodical title: Glycovirology
• Pages: 31-35
• Text Language Code: eng
• Subject Heading: Sialic acid; α2-3 linkage; α2-6 linkage; flow cytometry; MALII, SNA
• Target Audience: 一般
• Note (General): We described a method to detect α2-3 linked and α2-6 linked sialic acids on the cell surface with using flow cytometry. Cells were fixed with 4% paraformaldehyde, and then α2-3 and α2-6 sialic acids were stained with biotinylated MAACKIA AMURENSIS LECTIN II (MALII) and biotinylated ELDERBERRY BARK LECTIN (SNA), respectively. Sialic acids on the cell surface were cleaved by sialidase in acetate buffer at pH 5.5 to confirm the specificity of staining. Streptavidin conjugated with Alexa flour 488 was used to detect biotinylated lectins. Thus, the α2-3 linked and α2-6 linked sialic acids on the cell surface were semi-quantitatively detected by flow cytometry.; Methods in Molecular Biology book series (MIMB,volume 2556)Springer Protocols; source:https://doi.org/10.1007/978-1-0716-2635-1_3
• Format (IMT): application/pdf
• Access Restrictions: 限定公開
• Rights (production): © 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
• Data Provider (Database): 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• Original Data Provider (Database): 滋賀医科大学 : 滋賀医科大学機関リポジトリびわ庫

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